Disodium succinate

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study

Justification for type of information:

Experimental result performed using standard methods of analysis

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Principles of method if other than guideline:

This study was designed to assess the toxic effects of the test compound on the Zebra fish(Danio rerio) for the test material

GLP compliance:

not specified

Analytical monitoring:

not specified

Vehicle:

not specified

Details on test solutions:

The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 400 mg of the test substance in 4 liters of potable water (passed through reverse osmosis system) with continuous stirring for achieving test concentrations of 100 mg/L,respectively

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: Zebra fish
- Source:Anytime Pet, Jariphataka, Nagpur
- Length at study initiation : average 1.8 cm
- Weight at study initiation: 0.082g (average)
- Method of breeding: The fishes were kept in a static tank in tap water passed through reverse osmosis system, under natural conditions alongwith proper feed and aeration.
ACCLIMATION
- Acclimation period: 7 days
- Type and amount of food during acclimation:
- Feeding frequency during acclimation: Daily once
-Photoperiod: 16 hours light and 8 hours dark

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Post exposure observation period:

observations were made at 24, 48, 72 and 96 hours

Hardness:

155.5 mg of CaCO3

Test temperature:

23 °C

pH:

7.1

Dissolved oxygen:

7.0 mg/l

Nominal and measured concentrations:

100 mg/l

Details on test conditions:

TEST SYSTEM
- Test vessel: Bowl aquari
- fill space : 2 liter
- No. of organisms per vessel: 8 fishes


OTHER TEST CONDITIONS
- Adjustment of pH: 7.4
- Photoperiod:16 hours light and 8 hours dark

Reference substance (positive control):

no

Key result

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

mortality was not observed

Sublethal observations / clinical signs:

8.1. Mortality and symptoms:

Nominal Concentration (mg/l)	Number of fish		Mortality (cumulated) after
			3h		6h		24h		48h		72h		96h
			V1	V2	V1	V2	V1	V2	V1	V2	V1	V2	V1	V2
Control	4	4	-	-	-	-	-	-	-	-	-	-	-	-
100	4	4	-	-	-	-	-	-	-	-	-	-	-	-

Visible Symptoms:

Nominal Concentration (mg/l)

Number of fish

symptom (cumulated) after

3h

6h

24h

48h

72h

96h

V1

V2

V1

V2

V1

V2

V1

V2

V1

V2

V1

V2

Control

4

4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

100

4

4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

Explanation of symptoms:

A: apathy

N: Narcotic-like state

C: Swimming near water surface

M: Slow movements compared to control

NS: No abnormal symptoms

S: Surfacing behaviour

L: Loss of equilibrium

SM: Shimming movement

H1: Hyperactivity

H2: Hypo activity

B: Bending behavior

F: Fin rot

IS: Invert Swimming

SB: Swimming behaviour

RF: Respiratory Function

P: Pigmentation

pH and oxygen content:

Nominal Concentration (mg/l)	Number of fish		pH readings after
			24h		48h		72h		96h
			V1	V2	V1	V2	V1	V2	V1	V2
Control	4	4	7.3	7.3	7.3	7.5	7.5	7.5	7.6	7.6
100	4	4	7.4	7.4	7.5	7.5	7.7	7.7	7.8	7.8

Nominal Concentration (mg/l)	Number of fish		Oxygen content (mg/L) after
			24h		48h		72h		96h
			V1	V2	V1	V2	V1	V2	V1	V2
Control	4	4	7.0	7.0	6.9	6.9	6.7	6.7	6.6	6.6
100	4	4	6.9	6.9	6.8	6.8	6.7	6.7	6.6	6.6

Nominal Concentration (mg/l)	Number of fish		Temperature (°C) after
			24h		48h		72h		96h
			V1	V2	V1	V2	V1	V2	V1	V2
Control	4	4	23.2	23.2	23.4	23.4	23.9	23.9	23.9	23.9
100	4	4	23.2	23.2	23.4	23.4	23.9	23.9	23.9	23.9

Validity criteria fulfilled:

yes

Conclusions:

Based on nominal concentrations, experimental median lethal Concentrations [LC-50 (96 h)] for the test material on Zebra fish(Danio rerio)was found to be > 100 mg/L.

Executive summary:

Study was conducted to access the effect of test chemical on the growth of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test). The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 400 mg of the test substance in 4 liters of potable water (passed through reverse osmosis system) with continuous stirring for achieving test concentrations of 100 mg/L,respectively. Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. After 96 hours of exposure to test item to nominal test concentrations, LC50 was determine to be >100 mg/l . Based on the LC50, it can be consider that the chemical was toxic and can be consider to be not classified as per the CLP classification criteria.

Description of key information

Short term toxicity to fish:

Study was conducted to access the effect of test chemical on the growth of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test).The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 400 mg of the test substance in 4 liters of potable water (passed through reverse osmosis system) with continuous stirring for achieving test concentrations of 100 mg/L,respectively.Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. After 96 hours of exposure to test item to nominal test concentrations, LC50 was determine to be >100 mg/l . Based on the LC50, it can be consider that the chemical was toxic and can be consider to be not classified as per the CLP classification criteria.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

100 mg/L

Additional information

Short term toxicity to fish:

Toxicity of test material was evaluated on fish based on study report of the test material and data from authoritative database.Study was conducted to access the effect of test chemical on the growth of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test).The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 400 mg of the test substance in 4 liters of potable water (passed through reverse osmosis system) with continuous stirring for achieving test concentrations of 100 mg/L,respectively.Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. After 96 hours of exposure to test item to nominal test concentrations, LC50 was determine to be >100 mg/l . Based on the LC50, it can be consider that the chemical was toxic and can be consider to be not classified as per the CLP classification criteria.

In an authoritative data base , the short term toxicity test on fish was evaluated according to OECD guideline 203 . The LC50 value of the test substance to fish is found to be >100 mg/L. This value indicates that the substance is not likely to exhibit short term toxicity to fish.Based on the LC50, it can be consider that the chemical was toxic and can be consider to be not classified as per the CLP classification criteria.