Registration Dossier
Registration Dossier
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EC number: 250-333-2 | CAS number: 30772-79-3
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
Description of key information
A study was performed to assess the acute toxicity of the substance to Daphnia magna. The study was conducted in accordance with OECD Guideline for Testing of Chemicals No. 202 under static conditions.
Based on the results of a preliminary range-finding test, the following nominal concentrations were tested in the definitive test: 1.0, 1.8, 3.2, 5.6 and 10 mg/L
For each concentration and a control group, twenty Daphnia magna (less than 24 hours old) were exposed for 48 hours. The exposure took place in airtight closed vessels.The incidence of immobilisation was recorded for each test and control group at 24 hours and at 48 hours.
Samples taken from all concentrations at t = 0, 24 and 48 were analysed with a validated GC-FID method.
Analysis of the samples taken at the start of the final test showed that measured concentrations were lower than nominal. The concentrations remained stable during the 48-hour test period. Based on these results, the mean measured concentrations were calculated. The range tested based on mean measured concentrations was 0.67, 1.4, 2.5, 4.4 and 7.9 mg/L.
The 48h-EC50 value with Daphnia magna was 3.0 mg/L based on mean measured concentrations.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 3 mg/L
Additional information
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