Anilinoacetonitrile

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Meets generally accepted scientific standards, well documented and acceptable for assessment. No data on GLP and purity of test substance.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1976

Report date:

1976

Materials and methods

Principles of method if other than guideline:

Anilinoacetonitrile in a 0.5% suspension of carboxymethylcellulose was applied to 5 male and 5 female sprague dawley rats (per oral) at several doses. The observation period was 14 days. Weights were recorded before treatment and 2, 7, and 14 days after treatment. Clinical signs were recorded daily. Necropsy of animals that died was performed as early as possible. Necropsy of surviving animals was performed after 14 days.

GLP compliance:

not specified

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Anilinoacetonitrile

EC Number:

221-129-0

EC Name:

Anilinoacetonitrile

Cas Number:

3009-97-0

Molecular formula:

C8H8N2

IUPAC Name:

2-(phenylamino)acetonitrile

Test animals

Species:

rat

Strain:

Sprague-Dawley

Sex:

male/female

Administration / exposure

Route of administration:

oral: unspecified

Vehicle:

CMC (carboxymethyl cellulose)

Details on oral exposure:

Test substance formulation: 1.00, 1.47, 2.15, 3.16, 4.64, 6.81% test substance in 0.5% aquaeous carboxymethyl cellulose solution containing 2-3 drops Cremophor EL
Application volume: 10 ml/kg

Doses:

100, 147, 215, 316, 464, 681 mg/kg

No. of animals per sex per dose:

5

Control animals:

no

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

> 215 - < 316 mg/kg bw

Mortality:

Dose (mg/kg)
100: no deaths occured.
147: no deaths occured.
215: male: no deaths occured. female: 1 animal died within 2-7 days after application.
316: male: 5/5; animals died within 6-24 hours after application. female: no deaths occured.
464: male: 5/5; animals died within 4-24 hours after application. female: no deaths occured.
681: 5/5 male and female animals died within 24 hours.

Clinical signs:

other: Dose groups: 681: Dyspnea, apathy (until 2 days after Application). Lateral/abdominal/supine position (until 2 hours after application). Staggering until 15 min after application. Tonic-clonic convulsions (4 hours after application). Diarrhoe (1 hour afte

Gross pathology:

Phenylglycinenitrile: acute cardiac dilatation, partly acute medium graded pulmonary distension, partly conspicuous abundance of blood with light edemata, clay-grey liver with peripheral lobule marking