3-chlorobutan-2-one

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1975

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: non-GLP Study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1975

Report date:

1975

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 401 (Acute Oral Toxicity)

Principles of method if other than guideline:

Study performed before 1987.

GLP compliance:

not specified

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

3-chlorobutan-2-one

EC Number:

223-834-9

EC Name:

3-chlorobutan-2-one

Cas Number:

4091-39-8

Molecular formula:

C4H7ClO

IUPAC Name:

3-chlorobutan-2-one

Constituent 2

Reference substance name:

3-Chloro-2-butanone

IUPAC Name:

3-Chloro-2-butanone

Test material form:

other: coluorless liquid

Details on test material:

no info about substance purity in study report

Test animals

Species:

rat

Strain:

Wistar

Sex:

male

Details on test animals or test system and environmental conditions:

60 male Wistar rats bred at the institute were used for testing.
Bodyweight 205 +/- 14 g.
Animals were kept 16 hours before substance administration
without feed (drinking water ad libitum).

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

water

Remarks:

3% (v/v)

Details on oral exposure:

3-Chloro-2-butanone was applicated in a 3% (volume/volume) aqueous solution.
1 drop of Tween 40 was added per 100 mL solution. Oral (gavage) administration.

Doses:

Six dose groups: 0.900; 0.818; 0.744; 0.676; 0.615; 0.554 mL/kg

No. of animals per sex per dose:

10 animals (male) per dose (six dose groups)

Control animals:

not specified

Details on study design:

Mortality was monitored 24 hours and 14 days after substance application.

Statistics:

no data

Results and discussion

Effect levels

Sex:

male

Dose descriptor:

LD50

Effect level:

ca. 0.615 mL/kg bw

Based on:

test mat.

Mortality:

9/10; 6/10; 6/10; 6/10; 5/10; 1/10 (after 1 day)
9/10; 6/10; 6/10; 6/10; 5/10; 1/10 (after 14 day)

Clinical signs:

Clinical symptoms were observed - at all dose groups - 1-2 minutes after substance administration..
Symptoms were a lateral position, paralysis of skeletal muscles.
Laboured breathing occured dose depended; combined with pronounced abdominal breathing.

Body weight:

no data

Gross pathology:

no data

Other findings:

Mortality occured during the first day; the first animals died 4 hours after substance administration.
The animals surviving 14 days showed a reduced food and water consumption, course and
untended fur, curved back and reduced mobility.

Applicant's summary and conclusion

Interpretation of results:

Category 4 based on GHS criteria

Remarks:

Migrated information

Conclusions:

Classification as Toxicity Category IV.

Executive summary:

The LD50 dose after a single oral administration of 3-chloro-2-butanone

in male Wistar rats was found to be 0.615 mL/kg bw (14 days).