Diantimony trioxide

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro DNA damage and/or repair study

Remarks:

Type of genotoxicity: DNA damage and/or repair

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: The reference does not totally comply with the specific testing guideline: no positive controls or metabolic activation system were included. Due to the missing positive and negative control, the study was considered not reliable.

Data source

Reference

Reference Type:

publication

Title:

Rec assay and mutagenicity studies on metal compounds

Author:

Kanematsu N., Hara M. and Kada T.

Year:

1980

Bibliographic source:

Mutation Research, 77:109-116

Materials and methods

Test guideline

Guideline:

other: no guideline specified

Principles of method if other than guideline:

In this study where the genotoxicity of metal compounds were evaluated, antimony trioxide was tested in the Bacillus subtilis Rec assay .

GLP compliance:

not specified

Type of assay:

Bacillus subtilis recombination assay

Test material

Test material information

Constituent 1

Reference substance name:

Diantimony trioxide

EC Number:

215-175-0

EC Name:

Diantimony trioxide

Cas Number:

1309-64-4

Molecular formula:

Sb2O3

IUPAC Name:

oxostibanyl stibinate

Test material form:

not specified

Method

Species / strain

Species / strain / cell type:

bacteria, other: Bacillus subtilis H17 (Rec+, arg- try-) and M45 (Rec-, arg- try-)

Test concentrations with justification for top dose:

The antimony trioxide was dissolved in distilled water at a concentration of 0.001-10 M.
2.5 µmol (729 µg)/disk

Controls

Untreated negative controls:

no

Positive controls:

no

Details on test system and experimental conditions:

In this study the genotoxicity of 127 metal compounds were evaluated. One of the compounds was antimony trioxide that was tested in the Bacillus
subtilis Rec assay.
The strains were grown overnight in B-2 broth (meat extract 10g, polypeptone powder 10g, and NaCl 5 g in 1L, pH adjusted to 7.0) and conserved at -80°C with 12.5% glycerol supplemention. On the day of the experiment, each stock was melted and streaked radially from small pipettes onto
B-2 agar.
A 0.05 ml portion of the metal solution was dropped onto a filter paper disk (diameter 10 mm) and the disk was placed on the starting point of the
streak. The plates were kept at 4°C for 24 hours and then incubated at 37°C overnight.
When the DNA damage is produced by a chemical and subjected to cellular recombination-repair function, the growth of recombination-deficient
cells is usually inhibited much more than that of wild cells.

Evaluation criteria:

When a metal compound has the capacity to damage DNA, growth of the Rec- strain of Bacillus subtilis must be more inhibited than that of the wild
strain. To predict the DNA-damaging capacity, the inhibition zones were measured and compared.
A difference in the inhibition length of more than 4 mm was judged as positive.

Results and discussion

Additional information on results:

The treatment with antimony trioxide resulted in a difference in inhibition length of 5 mm and was thereby classified as positive.

Any other information on results incl. tables

Table 1: Results of the Rec assay on positive metal compounds

metal compound	concentration of solution (M)	inhibition length (mm)		difference	conclusion^a
		H17 (Rec^+)	M45 (Rec^-)
Sb2O3	0.05	0	5	5	+
^a+ Less than 6 mm and more than 4 mm of difference.

Applicant's summary and conclusion

Conclusions:

Interpretation of results (migrated information):
positive

The treatment with antimony trioxide resulted in a difference in inhibition length of 5 mm and was thereby classified as positive.