butyl methylphosphinate

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Remarks:

Type of genotoxicity: gene mutation

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1997

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1997

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 471 (Bacterial Reverse Mutation Assay)

GLP compliance:

yes

Type of assay:

bacterial reverse mutation assay

Test material

Reference

Name:

Unnamed

Type:

Constituent

Method

Species / strain

Species / strain / cell type:

S. typhimurium TA 1535, TA 1537, TA 98 and TA 100

Additional strain / cell type characteristics:

other: amino acid dependent

Metabolic activation:

with and without

Metabolic activation system:

rat liver homogenate

Test concentrations with justification for top dose:

4, 20, 100, 500, 2500 and 5000 μg/plate.

Vehicle / solvent:

DMSO (dimethyl sulfoxide)

Controls

Controls 1

Untreated negative controls:

yes

Negative solvent / vehicle controls:

yes

Remarks:

DMSO

True negative controls:

no

Controls 2

Positive controls:

yes

Positive control substance:

sodium azide

Remarks:

Strains TA 100 and TA 1535

Migrated to IUCLID6: without metabolic activation

Controls 3

Positive controls:

yes

Positive control substance:

9-aminoacridine

Remarks:

strain TA 1537

Migrated to IUCLID6: without metabolic activation

Controls 4

Positive controls:

yes

Positive control substance:

2-nitrofluorene

Remarks:

strain TA 98

Migrated to IUCLID6: without metabolic activation

Controls 5

Positive control substance:

other: 2-Aminotanthracene, withmetabolic activation

Remarks:

all strains

Details on test system and experimental conditions:

Two independent mutagenicity studies were conducted, each in the absence and in the presence of a metabolizing system derived from a rat liver homogenate. For both studies, the compound was dissolved in DMSO, and each bacterial strain was exposed to 6 dose levels. Doses for both studies ranged from 4 to 5000 μg/plate (4 / 20 / 100 / 500 / 2500 / 5000 μg/plate).

Results and discussion

Test results

Species / strain:

S. typhimurium TA 1535, TA 1537, TA 98 and TA 100

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

no cytotoxicity nor precipitates, but tested up to recommended limit concentrations

Vehicle controls validity:

valid

Untreated negative controls validity:

valid

Positive controls validity:

valid

Additional information on results:

Summarizing, it can be stated that the technical substance was not mutagenic in this bacterial mutation test either in the absence or in the presence of exogenous metabolic activation.

Remarks on result:

other: all strains/cell types tested

Remarks:

Migrated from field 'Test system'.

Applicant's summary and conclusion

Conclusions:

Interpretation of results (migrated information):
negative