2-chlorotoluene

Specific investigations: other studies

Administrative data

Endpoint:

specific investigations: other studies

Type of information:

experimental study

Adequacy of study:

other information

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

secondary literature

Cross-reference

Reason / purpose for cross-reference:

reference to same study

Data source

Reference

Reference Type:

secondary source

Title:

Unnamed

Year:

1974

Materials and methods

Principles of method if other than guideline:

Livers from 5 rats/group were obtained at the termination of the 14-day or 3-month segments of the subacute toxicity studies and from 4 dogs/group after treatment for 3 month.
The livers were weighed and liver/body weight (relative liver weight) ratios calculated. p-nitroanisole metabolism was determined according to the procedure employed by Kinoshita et al. 81966), Toxicol Appl. Pharmacol. 9, 505-513 and Hoffman et al. (1968), Toxicol Appl Pharmacol 12, 464-472

GLP compliance:

no

Type of method:

in vivo

Endpoint addressed:

not applicable

Test material

Test material information

1

Reference substance name:

2-chlorotoluene

EC Number:

202-424-3

EC Name:

2-chlorotoluene

Cas Number:

95-49-8

Molecular formula:

C7H7Cl

IUPAC Name:

1-chloro-2-methylbenzene

Test animals

Species:

other: rat and dog

Strain:

other: Harlan + Beagle

Sex:

male/female

Administration / exposure

Route of administration:

other: oral

Vehicle:

other: rat: unchanged dog: 5 % aqueous acacia solution

Analytical verification of doses or concentrations:

no

Duration of treatment / exposure:

14-days or 3-month

Frequency of treatment:

daily

Post exposure period:

no

Doses / concentrations

Doses / concentrations 1

Remarks:

Doses / Concentrations:
undilited
Basis:
other: rats

Doses / concentrations 2

Remarks:

Doses / Concentrations:
0, 5, 20 or 80 mg/kg bw/d in a 5 % aqueous accacia solution
Basis:
nominal conc.
dog

No. of animals per sex per dose:

rat: 20 males and 20 females/dose
dog: 2 males and 2 females/dose

Control animals:

yes, concurrent vehicle

Results and discussion

Details on results:

daily gavage administration of OCT to female rats for 14 days had no statistically significant effect on the rate of hepatic p-nitroanisole O-demethylation to p-nitriphenol. The rate of metabolism was increased significantly in male rats given 80 and 320 mg/kg/day, but at least a part of the difference between these rats and their respective controls was due to an unusually low rate of metabolism in the controls. Administration of OTC for 3 month had no significant effect on the rate of metabolism in either male or female rats.
Oral administration of OCT to dogs for 3 month had no effect on the rate of p-nitroanisole metabolism.

Any other information on results incl. tables

no data

Applicant's summary and conclusion

Executive summary:

Livers from 5 rats/group were obtained at the termination of the 14-day or 3-month segments of the subacute toxicity studies and from 4 dogs/group after treatment for 3 month.

The livers were weighed and liver/body weight (relative liver weight) ratios calculated. p-nitroanisole metabolism was determined.

daily gavage administration of OCT to female rats for 14 days had no statistically significant effect on the rate of hepatic p-nitroanisole O-demethylation to p-nitriphenol. The rate of metabolism was increased significantly in male rats given 80 and 320 mg/kg/day, but at least a part of the difference between these rats and their respective controls was due to an unusually low rate of metabolism in the controls. Administration of OTC for 3 month had no significant effect on the rate of metabolism in either male or female rats.

Oral administration of OCT to dogs for 3 month had no effect on the rate of p-nitroanisole metabolism.