Dimethyl methylphosphonate

Skin irritation / corrosion

Administrative data

Endpoint:

skin irritation: in vivo

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: scientifically acceptable study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1976

Report date:

1976

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

Draize test

GLP compliance:

no

Remarks:

well documented study

Test material

Test material information

Constituent 1

Reference substance name:

Dimethyl methylphosphonate

EC Number:

212-052-3

EC Name:

Dimethyl methylphosphonate

Cas Number:

756-79-6

Molecular formula:

C3H9O3P

IUPAC Name:

dimethyl methylphosphonate

Details on test material:

- Name of test material (as cited in study report): 80 021/B
No additional data

Test animals

Species:

rabbit

Strain:

other: Russian breed

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: rabbits (3 males; 3 females) of the Russian breed; no additional data
- Age at study initiation: no data
- Weight at study initiation: 1.7-2.0 kg
- Housing: housed singly in metal cages (overall dimensions 47x32x34 cm)
- Diet (e.g. ad libitum): standard diet of Nafag
- Water (e.g. ad libitum): drinking water
- Acclimation period: at least 8 days

ENVIRONMENTAL CONDITIONS
- Temperature (°C): 18±1°C
- Humidity (%): 55±5%
- Photoperiod (hrs dark / hrs light): 14 hours light/day

Test system

Type of coverage:

occlusive

Preparation of test site:

other: shaved (right side) and abraded (shaved skin area on the left side was slightly scarified just before treatment)

Vehicle:

unchanged (no vehicle)

Controls:

no

Amount / concentration applied:

TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 0.5 ml
- Concentration (if solution): 100%

Duration of treatment / exposure:

24 hours

Observation period:

7 days

Number of animals:

3 males and 3 females

Details on study design:

TEST SITE
- Area of exposure: a gauze patch of 2.5 x 2.5 cm laden with the test substance was applied immediately to the prepared skin
- % coverage: not specified
- Type of wrap if used: the patch was covered with an impermeable foil of 5 x 5 cm, which was fixed to the body of the animals with adhesive tape

REMOVAL OF TEST SUBSTANCE
- Washing (if done): not specified; the gauze patches were removed
- Time after start of exposure: 24 hours after application

SCORING SYSTEM: comparable to the OECD scoring system; the reaction of the skin was appraised upon removal during an observation period of 7 days.

Results and discussion

In vivo

Results

Results 1

Irritation parameter:

erythema score

Basis:

mean

Remarks:

of all tested animals

Time point:

other: 24-48-72-hour

Score:

0.28

Max. score:

4

Reversibility:

fully reversible within: 48 hours

Remarks on result:

other: Only shaved sites considered for evaluation; 5 animals (3 males and 2 females) showed an erythema score of each 1, reversible within 48 hours (see Table 1)

Results 2

Irritation parameter:

edema score

Basis:

mean

Remarks:

of all tested animals

Time point:

other: 24-48-72-hour

Score:

0

Max. score:

4

Reversibility:

other: not applicable

Remarks on result:

other: Only shaved sites considered for evaluation (see Table 2)

Irritant / corrosive response data:

see Table 1&2; a slight irritating effect was present on abraded sites (maximum score of 2 in only one animal after 24 hours), but fully reversible within 72 hours in all animals

Any other information on results incl. tables

Table 1: Erythema scores assuming (only shaved sites shown)

Time point	Individual and mean erythema scores
	Male animals			Male animals			Mean all animals
	1^st	2^nd	3^rd	4^th	5^th	6^th
24 h	1	1	1	1	0	1	0.83
48 h	0	0	0	0	0	0	0
72 h	0	0	0	0	0	0	0
Mean 24-48-72 h	0.3	0.3	0.3	0.3	0	0.3	0.28

 

Table 2: Edema scores (only shaved sites shown)

Time point	Individual and mean edema scores
	Male animals			Male animals			Mean all animals
	1^st	2^nd	3^rd	4^th	5^th	6^th
24 h	0	0	0	0	0	0	0
48 h	0	0	0	0	0	0	0
72 h	0	0	0	0	0	0	0
Mean 24-48-72 h	0	0	0	0	0	0	0