Benzeneacetic acid, 4-(cyclopropylcarbonyl)-.alpha.,.alpha.-dimethyl-, ethyl ester

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

Testing: 29 September- 17 October, 1997; Report: 7 January 1998

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP study performed according to international guidelines.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1998

Report date:

1998

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

4-(Cyclopropylcarbonyl)-α,α-dimethyl-ethyl ester benzeneacetic acid

EC Number:

605-546-9

Cas Number:

169280-10-8

Molecular formula:

C16 H20 O3

IUPAC Name:

4-(Cyclopropylcarbonyl)-α,α-dimethyl-ethyl ester benzeneacetic acid

Details on test material:

Batch: Dest. 3-1, dated April 7, 1997, from PT/TTF
Code: HOE CG 0530 OY ZD99 0001
Purity: 99%
Date of production: April 07, 1997
Date of expiry: April 1999
Storage conditions: darkness at approximately 5°C ina refrigerator

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

Samples (20 mL) were taken from the middle of the test solutions of 0, 10, 22 and 50 mg/L at the start, after 48 h and at the end of the 96-h test period. The samples were concentrated and resolved with mobile phase (acetonitrile) before measurement.

Test solutions

Vehicle:

no

Details on test solutions:

Test solutions were prepared using an Ultra-Torrax and quantitatively transferred to 4-litre test vessels. Then the different test solutions were stirred for 24h to ensure that the limit of solubility of the test substance in water was reached.

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

Day of hatching: May 15, 1997/ August 01, 1997
Day of delivery: June 9, 1997/ September 10, 1997
Fish were kept in dilution water 14 days before the start of the exposure.
Temperature: 22+/- 1°C
Oxygen content: >= 80 % of the saturation value
Light period: 12h daily
Population density: <= 1g fish/L water
Feeding: twice daily ad libitum
Food: Tetra Min, Tetra Werke, Melle (Germany)
Body length varied from 2.7 to 3.0 cm.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Post exposure observation period:

Not applicable.

Test conditions

Hardness:

2.3 - 2.7 mmol Ca2+ + Mg2+/L during the study.

Test temperature:

Controls (0 mg/L): 21.4 - 21.8 ºC
Test concentrations: 21.6 - 21.8 ºC

pH:

Controls (0 mg/L): 7.6- 8.2
Test concentrations: 7.6 - 8.3

Dissolved oxygen:

Controls (0 mg/L): 6.6 - 9.8 mg O2/L
Test concentrations: 7.0 - 9.4 mg O2/L

Nominal and measured concentrations:

Concentration (mg/L) calculated as geometric means:
Nominal Measured Recovery in %
10 4.8 48
22 13.4 61
50 16.7 33

Details on test conditions:

TEST SYSTEM
- Test chambers were calibrated to 4L and were not aerated during the course of the study.
- Chambers illumintated for 12h
- Light intensity: 700 lux
Allocation of fish: 7 fish in 3 group. First group: 0 and 50 mg/l, 2nd group: 0 and 22 mg/l, 3rd group: 0, 5 and 10 mg/l

TEST MEDIUM / WATER PARAMETERS
Reconstituted water, composition according to ISO/DIS 7346/1 was used as water for dilution. The dilution was aerated until oxygen saturation.
The pH was measured before the use of each batch. It was in the range of 8.0-8.3.

Reference substance (positive control):

not specified

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

4.8 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: Nominal concentration: 10 mg/L.

Effect concentrations 2

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 13.4 - < 16.7 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: Nominal range: 22 - 50 mg/L

Details on results:

Fish showed changes in appearance and behaviour only at the nominal concentgrations of 22 and 50 mg/L, where also lethality occurred.
Effect concentrations were already reached after 24 h of exposure and did not increase during the test period.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The 96h-LC50 of p-Cyclopropylketoester for fish is between 22 and 50 mg/L, based on nominal concentrations and is above the solubility limit of the test substance in the test water.