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EC number: 939-726-6 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 26 march to 10 may 1999
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Comparable to OECD 471 guideline but report is incomplete and the study is not GLP.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 001
- Report date:
- 2001
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- GLP compliance:
- no
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Reaction mass of 2-(2-thienyl)ethyl benzenesulphonate and toluene
- IUPAC Name:
- Reaction mass of 2-(2-thienyl)ethyl benzenesulphonate and toluene
- Details on test material:
- Batch: S3221 (8S 00038)
Constituent 1
Method
- Target gene:
- Histidine
Species / strain
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98, TA 100 and TA 102
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9-mix (containing 2.5% of S9) from livers of Aroclor 1254-treated rats
- Test concentrations with justification for top dose:
- Dose-range finding test (TA 98), plate incorporation and preincubation methods: 0, 50, 100, 500, 1000, 2500, 5000 µg/plate with and without metabolic activation.
Mutagenicity test, preincubation method: 0, 100, 500, 1000, 2500, 5000 µg/plate with and without metabolic activation. - Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: Dimethyl sulphoxide (DMSO)
Controlsopen allclose all
- Untreated negative controls:
- yes
- Remarks:
- DMSO (100µL/plate)
- Negative solvent / vehicle controls:
- yes
- Remarks:
- DMSO
- Positive controls:
- yes
- Remarks:
- Without metabolic activation
- Positive control substance:
- 9-aminoacridine
- 2-nitrofluorene
- sodium azide
- mitomycin C
- Remarks:
- sodium azide: 1µg/plate (TA100, TA1535); 2-nitrofluorene: 2.5 µg/plate (TA98); 9-aminoacridine: 100 µg/plate (TA1537); mitomycin C: 0.25 µg/plate (TA102)
- Untreated negative controls:
- yes
- Remarks:
- DMSO (100µL/plate)
- Negative solvent / vehicle controls:
- yes
- Remarks:
- DMSO
- Positive controls:
- yes
- Remarks:
- With metabolic activation
- Positive control substance:
- other: 2-aminoanthracene
- Remarks:
- 2-aminoanthracene: 1µg/plate (TA100); 2.5 µg/plate (TA98, TA1535, TA 1537); 5 µg/plate (TA102)
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: preincubation
Exposure duration: 48h (TA98, TA100, TA1535 and TA1537) and 72h (TA102)
Number of plates/concentration/test: 1 for the dose-range finding study, 3 for the mutagenicity test
Number of independent mutagenicity test: 1
Results and discussion
Test resultsopen allclose all
- Species / strain:
- S. typhimurium TA 98
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- from 2500 µg/plate upwards
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- positive
- Remarks:
- significant increase in the number of revertants more pronounced without S9-mix and a clear dose-related effect with and without S9-mix
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- at 5000 µg/plate
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 102
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- at 5000 µg/plate
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 1535
- Metabolic activation:
- with and without
- Genotoxicity:
- positive
- Remarks:
- significant increase in the number of revertants more pronounced without S9-mix and a clear dose-related effect with and without S9-mix
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- from 500 µg/plate upwards with S9-mix and from 2500 µg/plate upwards without S9-mix
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 1537
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- at 5000 µg/plate
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Additional information on results:
- RANGE-FINDING/SCREENING STUDIES: Precipitation was noted from 1000 µg/plate upwards without S9-mix and with the plate incorporation method and at 5000 µg/plate with S9-mix and the preincubation method.
No toxic effects were noted with the plate incorporation method whether with or without S9-mix or with the preincubation method with S9-mix; only moderate toxicity was reported at 5000 µg/plate without S9-mix with the preincubation method. Consequently, 5000 µg/plate was chosen as the top dose to conduct the mutagenicity study as it is the maximum dose recommended by international regulations and the preincubation method was selected since it is considered to be more sensitive.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
positive
BTE in toluene showed a mutagenic activity with and without S9-mix in the bacterial reverse mutation test in TA 1535 and TA 100, two Salmonella typhimurium strains that detect base-pair substitution mutagens.
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