Registration Dossier
Registration Dossier
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EC number: 223-276-6 | CAS number: 3806-34-6
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
Description of key information
Key value determined in GLP accredited laboratory test to OECD Guideline 201 and EU Method C.3.
The value is based on nominal loading rates.
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 100 mg/L
- EC10 or NOEC for freshwater algae:
- 50 mg/L
Additional information
A study was performed to assess the effect of the test item on the growth of the green alga Pseudokirchneriella subcapitata.
Due to the low aqueous solubility and complex nature of the test item for the purposes of the test the test item was prepared as a Water Accommodated Fraction (WAF).
Following a preliminary range-finding test, Pseudokirchneriella subcapitata was exposed to Water Accommodated Fractions (WAFs) of the test item over a range of nominal loading rates of 6.25, 12.5, 25, 50 and 100 mg/L (three replicate flasks per concentration) for 72 hours, under constant illumination and shaking at a temperature of 24 ± 1 °C.
Results
Exposure of Pseudokirchneriella subcapitata to the test item gave the following results:
ErL50 (0 – 72 h): >100 mg/L loading rate WAF
EyL50 (0 – 72 h): >100 mg/L loading rate WAF
NOELR: 50 mg/L
LOELR: 100 mg/L
Analysis of the test preparations at 0 hours showed measured test concentrations to range from 3.2 to 55 mg/L whilst concentrations in the range of 1.5 to 55 mg/L were obtained at 72 hours.
Given that the toxicity cannot be attributed to a single component or a mixture of components but to the test item as a whole, the results were based on nominal loading rates only.
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