Disodium 5-[[4-[(2-bromo-1-oxoallyl)amino]-2-sulphonatophenyl]azo]-4-hydroxy-6-(methylamino)naphthalene-2-sulphonate

Biodegradation in water: screening tests

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

biodegradation in water: screening test, other

Type of information:

experimental study

Adequacy of study:

key study

Study period:

06 May 1993 to 25 June 1993

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

test procedure in accordance with national standard methods

Qualifier:

according to guideline

Guideline:

ISO 5815 (Water quality - Determination of Biochemical Oxygen Demand after 5 Days (BOD5) - Dilution and Seeding Method)

Deviations:

yes

Remarks:

Aerate over night after addition of the inoculum

GLP compliance:

yes (incl. QA statement)

Remarks:

OECD Good Laboratory Regulations and the Procedures and Principles of Good Laboratory Praxis in Switzerland

Specific details on test material used for the study:

SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: 287380.26
- Expiration date of the lot/batch: Nov 1997

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: room temperature
- Solubility of the test substance in the solvent/vehicle: 50 g/l (in water)

Oxygen conditions:

aerobic

Inoculum or test system:

sewage, domestic (adaptation not specified)

Details on inoculum:

- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Seeding water of an urban waste water
- Concentration of sludge: 6 to 800 mg/l

Duration of test (contact time):

5 d

Initial conc.:

> 6.5 - < 827.9 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation:

O2 consumption

Details on study design:

TEST CONDITIONS
- Test temperature: 20 ± 1 °C

Reference substance:

other: D(+) - Glucose (Merck Nr. 8337) / L-Glutamic acid (Merck Nr. 291)

Remarks:

tested in a concentration of 20 ml/l.

Preliminary study:

None

Test performance:

None

Remarks on result:

other: data not available.

Parameter:

BOD5

Value:

11 mg O2/g test mat.

Results with reference substance:

The BOD5 of the reference substance is 205 mg O2/I.

Validity criteria fulfilled:

yes

Interpretation of results:

other: BOD5

Conclusions:

The BOD5 of FAT 92354/A is 11 mg O2/g.

Executive summary:

The determination of the BOD₅ was conducted according to ISO 5815 Second Edition 1989-08-01 (E) guideline and in accordance with GLP. The biochemical oxygen demand of FAT 92354/A is determined by measuring the dissolved oxygen concentration before and after 5 days incubation at 20 °C in different test concentrations diluted in water saturated with dissolved oxygen that contains a seed of microorganisms. The test organism was collected from a seeding water of urban waste water.

The biological oxygen demand (BOD5) of FAT 92354/A was evaluated in the current study. The test substance was tested in concentrations from about 6 to 800 mg/l, while the reference substance D(+)-Glucose / L-Glutamic acid was tested in a concentration of 20 ml/l.

Based on the findings of the study, the BOD₅ of FAT 92354/A is 11 mg O₂/g while of the reference substance is 205 mg O₂/l.

Reference 2

Endpoint:

biodegradation in water: screening test, other

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1993

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

other: EEC Directive 84/449 (1984), Part. C.9 according to DEV H 41-1, Deutsches Einheits-Verfahren, 1980

Deviations:

yes

Remarks:

Mean of 2 determinations instead of 3

GLP compliance:

yes (incl. QA statement)

Remarks:

OECD Good Laboratory Regulations and the Procedures and Principles of Good Laboratory Praxis in Switzerland.

Specific details on test material used for the study:

SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: 287380.26
- Expiration date of the lot/batch: Nov 1997

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: room temperature
- Solubility of the test substance in the solvent/vehicle: 50 g/l (in water)

Oxygen conditions:

aerobic

Inoculum or test system:

not specified

Details on inoculum:

Not applicable

Duration of test (contact time):

2 h

Initial conc.:

5 mg/L

Based on:

test mat.

Details on study design:

None

Reference substance:

other: Potassium hydrogen phthalate solution (Merck-Nr. 11769)

Remarks on result:

other: data not available.

Parameter:

COD

Value:

742 g O2/g test mat.

Results with reference substance:

The COD of the reference substance is 198 mg O2/I.

Validity criteria fulfilled:

yes

Interpretation of results:

other: COD determined

Conclusions:

The COD of FAT 92354/A is 742 mg O2/g.

Executive summary:

The purpose of the study was to determine COD of the test substance as per EEC Directive 84/449 (1984), Part. C.9 according to DEV H 41-1, Deutsches Einheits-Verfahren, 1980 and in accordance with GLP. A pre-determinated amount of the test substance (FAT 92354/A) dissolved in water is oxidized by potassium dichromate in a strong sulphuric acid medium with silver sulphate as a catalyst under reflux for 2 hours. The residual dichromate is determined by titration with standardized ferrous ammonium sulphate. The test substance concentration was tested in 5.0 mg/l. The reference substance was potassium hydrogen phthalate solution was tested in a concentration of 20 ml/l. Based on the findings of the study, the COD of FAT 92354/A is 742 mg O₂/g while of the reference substance is 198 mg O₂/l.

Reference 3

Endpoint:

biodegradation in water: inherent biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

12 May 1993 to 17 September 1993

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 302 B (Inherent biodegradability: Zahn-Wellens/EMPA Test)

Deviations:

no

Principles of method if other than guideline:

Guideline followed

GLP compliance:

yes (incl. QA statement)

Remarks:

OECD Good Laboratory Regulations and the Procedures and Principles of Good Laboratory Praxis in Switzerland

Specific details on test material used for the study:

SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: 287380.26
- Expiration date of the lot/batch: Nov 1997

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: room temperature
- Solubility of the test substance in the solvent/vehicle: 50 g/l (in water)

Oxygen conditions:

aerobic

Inoculum or test system:

other: biological sewage treatment plant

Details on inoculum:

Strain / Inoculation: Sludge of a biological sewage treatment plant (ARA-Therwil) collected on 01.06.93

Duration of test (contact time):

28 d

Initial conc.:

150 mg/L

Based on:

DOC

Parameter followed for biodegradation estimation:

DOC removal

Details on study design:

Water / Nutrient: according to the specification of the EEC L133 (pp. 99-105) Volume 31 resp. OECD Method 302B.
Temperature: 22.0 ± 3 °C (room temperature)
Test duration: 28 days
Lighting: indirect daylight

Reference substance:

diethylene glycol

Parameter:

% degradation (DOC removal)

Value:

0

Sampling time:

28 d

Details on results:

Test substance:
The bioelimination of FAT - 92354/A measured as DOC after 28 days corrected by the blank control is: 0 % (average of 2 tests running in parallel)
Adsorption after 3 hours: 5.6 %

Results with reference substance:

The bioelimination, corrected by the blank control and measured as DOC (mg/l) is: 99.3 % 26th day

Validity criteria fulfilled:

yes

Interpretation of results:

not inherently biodegradable

Conclusions:

The bioelimination of FAT 92354/A observed after 28 days was 0 %.

Executive summary:

The inherent biodegradability of FAT 92354/A was determined in a 28 days Zahn-Wellens - Test according to OECD Guideline for Testing of Chemicals, No. 302B, Paris 1981 and the EEC-Directive, L133 (pp. 99-105) Volume 31, May 88. The test was performed in compliance with the Good Laboratory Practice (GLP) Regulations of Switzerland. The test substance was tested in concentration of 150 mg/l DOC. The reference substance diethylene glycol was also tested in a concentration of 150 mg/l DOC. Based on the study results, the bioelimination of the test substance (FAT 92354/A) after 28 days was 0 % while of the reference substance after 26 days was 99.3 %.

Description of key information

In the studies designated as key, the BOD₅ of FAT 92354/A was 11 mg O₂/g, while the COD of FAT 92354/A was 742 mg O₂/g. The resulting BOD₅/COD ratio of 0.015 indicates that this test substance (FAT 91354/A) is not readily biodegradable. In a second study, the BOD₅ of FAT 40063/A was 64 mg O₂/g, while the COD was 696 mg O₂/g and the resulting BOD₅/COD calculation which comes to 0.1 also indicates that this test substance (FAT 40063/A) is not biodegradable. The bioelimination of the test substance (FAT 92354/A) after 28 days was 0 % in Zahn Wellens study, while in a second study, the bioelimination of the test substance (FAT 40063/A) after 31 days was 37 %. As a conclusion, the substance is considered to be not readily biodegradable.

Key value for chemical safety assessment

Biodegradation in water:

under test conditions no biodegradation observed

Type of water:

freshwater