[1,1'-Biphenyl]-2-carbonitrile, 4'-[(2-butyl-4-oxo-1,3-diazaspiro[4.4]non-1-en-3-yl)methyl]-

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

27 September 1996 to 21 November 1996

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Guideline study; GLP compliance

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1996

Report date:

1996

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Deviations:

not specified

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

4'-((2-butyl-4-oxo-1,3-diazaspiro[4.4]non-1-ene-3-yl)methyl)(1,1'-biphenyl)-2-carbonitrile

EC Number:

604-075-6

Cas Number:

138401-24-8

Molecular formula:

C25H27N3O

IUPAC Name:

4'-((2-butyl-4-oxo-1,3-diazaspiro[4.4]non-1-ene-3-yl)methyl)(1,1'-biphenyl)-2-carbonitrile

Details on test material:

- Code of test material (as cited in study report): SR47563
- Chemical name:4’-[(2-butyl-4-oxo-1,3-diaza-spiro[4.4]non-1-en-3-yl)methyl]- biphenyl]-2-carbonitrile
- Physical state: white-beige powder
- Lot/batch No.: 5ARL005
- Solubility: 1.3E-3 g/L at 23˚C
- Stability: stable under normal conditions of use
- Moll mass: 385.5
- Molecular formular: C25H27N3O
- Melting point: 97˚C

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Daphnia magna

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 1.08 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

1.08 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

test mat.

Basis for effect:

mobility

Any other information on results incl. tables

Test concentrations measured at the start of the test: 0.23; 0.28; 0.37; 0.42; 0.49; 0.59; 0.61; 0.82; 1.08 mg/l. During the test, pH varied between 7.3 to 7.8. The test concentrations decreased by 5.4 to 38.1 % during the 48 -h test period.

Applicant's summary and conclusion

Validity criteria fulfilled:

no

Remarks:

The criterion with respect to the stability of the test concentrations (decrease not > 20%) was not fulfilled in 4 out of the 9 tested. All other validity criteria had been fulfilled.

Conclusions:

The EC50 for mobility could not be determined at either 24 or 48 h of exposure, because no immobility of Daphnia magna was recorded at concentrations up to the maximum solubility of the test substance in water (ca. 1 mg/L). Although concentrations measured were not consistently stable during the test period, the substance appears to induce no acute effect on the mobility of the invertebrates exposed at or below its solubility in water.