Registration Dossier
Registration Dossier
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EC number: 940-123-5 | CAS number: 866889-74-9
Endpoint summary
Administrative data
Description of key information
Additional information
Biodegradation in water: screening test
The biodegradation of C16-18 DMAPA amidoamine (100% a.i.) was investigated in a study conducted according to OECD Guideline 301 F, adopted 17th July 1992 (Ready Biodegradability: Manometric Respirometry Test) and EU Method C.4-D, December 1992, over a period of 28 days using activated sludge sampled from a sewage treatment plant mainly fed with municipal wastewater as inoculum. The biodegradation rate was determined by measurement of O2 consumption. Inoculum blank, toxicity control and procedural/functional control with the reference substance Sodium benzoate were performed. The test was performed with 16 and 100 mg/L test substance concentration. The functional control reached the pass level >60% after 14 d (91%).
The biodegradation of the test substance was 78% (at 16 mg/L) and 85% (at 100 mg /L) after 28 days. The 10-day window started at day two and three, respectively. The biodegradation within the 10-day-window was 69% and 77%, respectively.
In the toxicity control the biodegradation was found to be 80% after 14 days of incubation. Thus, the demanded threshold value of 25% is exceeded and the test item can be identified as not inhibitory to microorganisms.
100 mg test item correspond to 316 mg ThOD, which is clearly above the demanded value of at least 50-100 mg ThOD/L. Therefore, an additional assay with 16 mg test item (51 mg ThOD, therefore, still meeting the required concentration of 50-100 mg ThOD/L) was conducted. At a concentration of 16 mg/L, the test item obtained a biodegradation above 60% (78%) within the test duration.
In this test, C16-18 DMAPA amidoamine was readily biodegradable fulfilling the 10-day criterion.
Biodegradation in water, soil and sediment:
According to REACH regulation, Annex IX, 9.2.1.2, 9.2.1.3 and 9.2.1.4, column II, the biodegradation simulation test in water, soil and sediment does not need to be conducted if the substance is readily biodegradable.
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