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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Toxicity to microorganisms

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Administrative data

Link to relevant study record(s)

Reference
Endpoint:
toxicity to microorganisms, other
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
comparable to guideline study
Principles of method if other than guideline:
The effect of test substance was studied on Tetrahymena pyriformis.
GLP compliance:
no
Analytical monitoring:
no
Vehicle:
not specified
Test organisms (species):
Tetrahymena pyriformis
Details on inoculum:
Cultures are reared in 50 m L of a sem i -defined medium 250-m L Erlenmeyer flasks.
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
40 h
Test temperature:
27±1°C
Nominal and measured concentrations:
Nominal 5 concentrations were taken
Key result
Duration:
40 h
Dose descriptor:
other: ICG 50
Effect conc.:
0.18 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth inhibition
Remarks on result:
other:
Remarks:
Not known
Validity criteria fulfilled:
not specified
Conclusions:
The effect of the test chemical was studied on the Tetrahynema pyriformis, the ICG50 was determined to be 0.18 mg/L
Executive summary:

The study was conducted to evaluate the toxic effects of the test chemical on the microorganism Tetrahynema pyriformis. The test was conducted for the period of 40-h in static conditions. Five different concentrations were taken to evaluate the ICG 5, median inhibition growth concentration. Test was conducted in the replicates and the growth inhibition was recorded using spectrophotometrically.Based on the results obtained from the experiment the ICG 50 was determined as 0.18 mg/L

Description of key information

The study was conducted to evaluate the toxic effects of the test chemical on the microorganism Tetrahynema pyriformis. The test was conducted for the period of 40-h in static conditions. Five different concentrations were taken to evaluate the ICG 5, median inhibition growth concentration. Test was conducted in the replicates and the growth inhibition was recorded using spectrophotometrically.Based on the results obtained from the experiment the ICG 50 was determined as 0.18 mg/L

Key value for chemical safety assessment

EC50 for microorganisms:
0.18 mg/L

Additional information

Data from the peer reviewed journal states that, The study was conducted to evaluate the toxic effects of the test chemical on the microorganism Tetrahynema pyriformis. The test was conducted for the period of 40-h in static conditions. Five different concentrations were taken to evaluate the ICG 5, median inhibition growth concentration. Test was conducted in the replicates and the growth inhibition was recorded using spectrophotometrically. Based on the results obtained from the experiment the ICG 50 was determined as 0.18 mg/L