[No public or meaningful name is available]

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Study period:

08.04.2021 - 09.07.2021

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2021

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)

Test guideline 2

Qualifier:

according to guideline

Guideline:

other: Commission Regulation (EC) No 2016/266, C.3

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Reaction Products of 1,1,1-Trimethylol-propane-triacrylate with aziridine

Molecular formula:

C14H24O5N - C47H77O16N4

IUPAC Name:

Reaction Products of 1,1,1-Trimethylol-propane-triacrylate with aziridine

Test material form:

liquid: viscous

Specific details on test material used for the study:

Name of test substance: Emuldur 3643
Test substance No.: 19/0071-2
Batch identification: 27771924U0
Purity: 100 area-% UVCB

Sampling and analysis

Analytical monitoring:

yes

Test organisms

Test organisms (species):

Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)

Details on test organisms:

TEST ORGANISM
- Common name: green alga
- Strain: Pseudokirchneriella subcapitata (Reinsch) Korshikov (syn. Selenastrum capricornutum Prinz)
- Source (laboratory, culture collection): stock obtained from the SAG (Collection of algal cultures in Göttingen, Germany)
- Age of inoculum (at test initiation): 4 days

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

72 h

Test conditions

Test temperature:

22.1 - 22.2°C

pH:

7.9 - 8.1

Nominal and measured concentrations:

Control; 0.032, 0.1, 0.32, 1, 3.2, 10 and 32 mg/L (nominal), corresponding to geometric mean measured concentrations of control, 0.03, 0.10, 0.31, 0.96, 3.05, 9.54 and 31.25 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: Erlenmeyer flasks (nominal volume 250 mL)
- Type: plugged with gas permeable silicone sponge caps
- Shaking rate: continuous at 85 rpm

- Initial cells density: 0.5 x 10^4 cells/mL
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 6

GROWTH MEDIUM
- Source/preparation of dilution water: preparation according to OECD Guideline for Testing of Chemicals, No. 201 Algal growth inhibition test
- pH: 7.9 - 8.1

OTHER TEST CONDITIONS
- Sterile test conditions: yes
- Photoperiod: 24 hours light per day
- Light intensity and quality: 6699 - 7210 lux at 400 - 700 nm

Results and discussion

Effect concentrations

Effect concentrations 1

Key result

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

1.639 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Effect concentrations 2

Duration:

72 h

Dose descriptor:

EC10

Effect conc.:

0.098 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Effect concentrations 3

Key result

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

0.032 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

In a 72-hour algae test with Pseudokirchneriella subcapitata, the 72 h EC50 value for Emuldur 3643 was determined to be 1.639 mg/L. The NOEC was determined 0.032 mg/L.