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Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
November 2020 to May 2021
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
GLP compliance:
yes (incl. QA statement)
Specific details on test material used for the study:
Batch Number CH-DA-01-18
Analytical monitoring:
no
Vehicle:
no
Details on test solutions:
Preparation and application of test solution
-Method: Water Accommodated Fractions
Test organisms (species):
Daphnia magna
Details on test organisms:
Sourced from ephippia supplied by Cheshire Eco Solutions, from MicroBioTests. The ephippia were incubated no more than 96h prior to test commencement, therefore the neonates were less than 24h old at the test commencement.
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Remarks on exposure duration:
Observations of the test animals were carried out at 24h and 48h for the definitive test.
Test temperature:
20 oC +/- 2 oC
pH:
6-9
Dissolved oxygen:
> 3 mg/L
Nominal and measured concentrations:
Nominal concentrations: 10, 18, 32, 56, 100, 180, 320, 560 and 1000 mg/L
Details on test conditions:
TEST SYSTEM
-Test Vessel: 100 mL capacity borosilicate glass crystallising dishes
-Type: Closed
-Volume of solution: 50 mL
-Type of flow-through: Static
-No. organisms per vessel: 10 animals per vessle
-No. vessels per concentration (replicates): 2 vessels per concentration
-No. vessels per control (replicates): 2 vessels per control

TEST MEDIUM/WATER PARAMETERS
-Source/preparation of dilution water: ISO reconstituted water (as specified in OECD 2020)
-Intervals of water quality measurement carried out at each concentration at 0h and 48 h

EFFECT PARAMETERS MEASURED: Immobilisation

OTHER TEST CONDITIONS
-Photoperiod: 16h light / 8h dark

RANGE FINDING STUDY
-Test concentrations: 1,000, 100, 10 and 1 mg/L
-Results used to determine conditions for the definitive study: yes
Reference substance (positive control):
yes
Remarks:
Potassium dichromate
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
56 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
212 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
274 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
Control mortality was 2.5%
Results with reference substance (positive control):
-Results with reference substance valid? Yes
-Relevant effect levels: yes
-Limit test: no
Dose-response test: yes
24h EC50: 1.4 mg/L























































































Nominal Conentration (mg/L)Number immobile at 24hNumber immobile at test termination (48h)Total exposed
Replicate aReplicate bReplicate aReplicate b
10000020
18000020
32000020
56102120
100002220
180223520
320678920
5601010101020
10001010101020
Validity criteria fulfilled:
yes
Conclusions:
The 48-hour EC 50 was 212 mg/L and the 48-hour NOEC was 56 mg/L. The substance does not meet the criteria for classification as toxic to the aquatic environment according to Regulation (EC) 1272/2008.
Executive summary:

The short-term toxicity of dihydronepetalactone to aquatic invertebrates was determined during a GLP-compliant study performed according to OECD Testing Guideline 202.


Since the substance was poorly water soluble, Water Accomodated Fractions (WAF) were prepared.  Results are expressed on the initial loading of the test substance required to produce effects.


The species was Daphnia magna.


A range-finding test was performed to determine the concentrations to be used during the definitive test.  As a result, concentrations of 10, 18, 32, 56, 100, 180, 320, 560 and 1000 mg/L were used during the definitive test.


The 48-hour EC50 was 212 mg/L and the 48-hour NOEC was 56 mg/L.  The substance did not meet the criteria for classification as toxic to the aquatic environment according to Regulation (EC) 1272/2008.

Description of key information

The short-term toxicity of dihydronepetalactone to aquatic invertebrates was determined during a GLP-compliant study performed according to OECD Testing Guideline 202.


Since the substance was poorly water soluble, Water Accomodated Fractions (WAF) were prepared.  Results are expressed on the initial loading of the test substance required to produce effects.


The species was Daphnia magna.


A range-finding test was performed to determine the concentrations to be used during the definitive test.  As a result, concentrations of 10, 18, 32, 56, 100, 180, 320, 560 and 1000 mg/L were used during the definitive test.


The 48-hour EC50 was 212 mg/L and the 48-hour NOEC was 56 mg/L.  The substance did not meet the criteria for classification as toxic to the aquatic environment according to Regulation (EC) 1272/2008.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Dose descriptor:
EC50
Effect concentration:
212 mg/L

Additional information