(1S,4R)-1-methyl-4-(prop-1-en-2-yl)cyclohex-2-en-1-ol

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2019-07-29 to 2019-09-02

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2019

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)

Version / remarks:

1992

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

(1S,4R)-1-methyl-4-(prop-1-en-2-yl)cyclohex-2-en-1-ol

Cas Number:

22972-51-6

Molecular formula:

C10H16O

IUPAC Name:

(1S,4R)-1-methyl-4-(prop-1-en-2-yl)cyclohex-2-en-1-ol

Test material form:

liquid

Details on test material:

Test item: (1S,4R)-1-Methyl-4-(prop-1-en-2-yl)cyclohex-2-enol
CAS number: 22972-51-6
Physical state: Liquid
Storage: Room temperature

Specific details on test material used for the study:

Test item: (1S,4R)-1-methyl-4-(prop-1-en-2-yl)cyclohex-2-en-1-ol (PMDOL)
CAS number: 22972-51-6
Physical state: Liquid
Storage: room temperature

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, non-adapted

Details on inoculum:

- Source: The inoculum was derived from the secondary effluent of
Tamsui Sewage Treatment Plant. Collect fresh sample and keep it aerobic
during transport. The inoculum was used as 100 mL /L in mineral medium.
-Test condition: The test groups were stirred in air-tight flasks, and the
measurement was in dark at 21 ± 1℃.
-The inoculum was pre-conditioned by aerating the secondary effluent, without
other treatment or addition, for 7 days at the test temperature

Duration of test (contact time):

28 d

Initial test substance concentration

Initial conc.:

100 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

O2 consumption

Details on study design:

Preparation of test vessels :
-Test article: 20 mg test article+20 mL inoculum+180 mL mineral medium
-Inoculum blank: 20 mL inoculum+180 mL mineral medium
-Procedure control: 20 mg reference article+20 mL inoculum+180 mL
mineral medium
-Toxicity control: 20 mg test article+20 mg reference article+20 mL inoculum
+180 mL mineral medium

Reference substance

Reference substance:

acetic acid, sodium salt

Results and discussion

% Degradation

Key result

Parameter:

% degradation (O2 consumption)

Value:

77

Sampling time:

28 d

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

readily biodegradable

Conclusions:

This study following the test guideline of OECD 301 F was to evaluate the possibility of test article consumed by microorganisms under aerobic conditions.
At the end of the test, the average BOD score of test article “Menthadienol (PMDOL)” was 2.20 (mg O2/mg). The percentage biodegradation reaches 77.46% of ThOD within 28 day period in a 10-day window.
Under the conditions of this study, the test article met the requirements of the ready biodegradability.