2,2,3,3,4,4,5,5,6,6,7,7-dodecafluoroheptan-1-ol

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2020

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Details on sampling:

The samples, both validation samples and biological samples, were directly injected and analysed by GC.

Vehicle:

no

Details on test solutions:

To produce the different test item concentrations appropriate amounts of the stock solution were diluted with dilution water to a volume of 500 mL. 50 mL of the prepared solutions were taken per replicate and 0.5 mL of dilution water containing 5 daphnids was given to all replicates resulting in the final nominal concentrations. For each test item exposure concentration and for the control exposure 4 replicates were prepared.

Test organisms (species):

Daphnia magna

Details on test organisms:

Daphnia magna STRAUS, parthenogenetic females, Source : Strain of Bundesgesundheitsamt Berlin

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Hardness:

The total hardness of the dilution water, measured at test start, was 14.7 °dH (= 262 mg/L CaCO3). The culture and dilution water was prepared and maintained within the specified pH range (6-9), while the hardness fell only slightly outside of the recommended range
(140 – 250 mg/L as CaCO3) specified by the OECD test guideline.

Test temperature:

20 +/- 2 °C

pH:

7.9

Dissolved oxygen:

8.8-9.0 mg/l

Nominal and measured concentrations:

The results are expressed in terms of geometric mean measured concentrations. Measured test item exposure concentrations ranged from 41.6 % to 78.6 % of their nominal target values at 0 hours and from 41.6 % to 49.2 % of their nominal target values at 48 hours.

Details on test conditions:

Test vessels : 100 mL glass beakers covered with watch glasses holding 5 neonates in 50 mL test medium
Experimental design : 9 test concentrations plus 1 control
5 neonates per vessel, 4 replicates per concentration/control
no feeding during the exposure period
static system
Method of initiation : neonates were placed in prepared media
Photoperiod : 16 h light : 8 h dark
Temperature of incubation unit : 19.7 to 20.0 °C

Reference substance (positive control):

no

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

27 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

The Daphnia were exposed to a range of concentrations, nominally 0.16, 0.31, 0.63, 1.25, 2.5, 5, 10, 20 and 40 mg/L of 1H,1H,7H-perfluoroheptane-1-ol dissolved in dilution water. Auxiliaries used to prepare the test media was a magnetic stirrer.
Observations were made on the swimming ability and the percentage immobilised, respectively, after 24 and 48 hours of exposure.

Validity criteria fulfilled:

yes

Conclusions:

The substance has an EC50 of 27 mg/l (based on mobility) towards daphnia magna, based on an OECD 202 guideline study.

Executive summary:

The substance has an EC50 of 27 mg/l (based on mobility) towards daphnia magna, based on an OECD 202 guideline study.

Description of key information

The substance has an EC50 of 27 mg/l (based on mobility) towards daphnia magna, based on an OECD 202 guideline study.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

27 mg/L