Tetrakis(1-piperidyl)phosphonium chloride

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

other information

Study period:

January 31st, 2000 - February 4th, 2000

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2000

Report date:

2000

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

Version / remarks:

1992

Deviations:

no

Test guideline 2

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Version / remarks:

1992

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Test material form:

solid

Specific details on test material used for the study:

Purity: 95.6 % [w/w]

Sampling and analysis

Analytical monitoring:

yes

Remarks:

The values determined were in a range of +/- 10% of the theoretical values.

Test solutions

Vehicle:

no

Details on test solutions:

Reconstitute water, composition according to ISO/DIS 7346/1 was used as water for dilution.
The test substance was weighed into a beaker. Water for dilution was added, the mixture was homogenized using an Ultra-Turrax and poured into the test chamber under stirring with a glass rod.
In order to determine the substance concentration, water samples were taken from the middle of the test chamber at study start, after 48 and 96 hours.

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

Origin: Bio International B.V., Postbus 63, NL-5845 ZH St. Anthonis, The Netherlands
Body length: 2.5 - 2.8 cm, mean 2.6 cm, s = +/- 0.12 cm
Feeding: twice daily ad libitum with Tetra Min
Fish density: ≤1 g fish / L water

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Test temperature:

21.6 - 21.9°C

pH:

Before Start and at 0h: 8.1 - 8.2
At 24, 48, 72 and 96h: 7.6 - 8.0

Dissolved oxygen:

Before Start and at 0h: 9.5 - 10.1 mg/L
At 24, 48, 72 and 96h: 7.2 - 8.1 mg/L
Oxygen saturation (100 %) at 22°C and local atm. pressure: 8.8 - 8.9 mg/L

Nominal and measured concentrations:

Nominal: 0, 10, 22, 50, 100 and 220 mg/L

Details on test conditions:

After the test concentrations had been prepared and water parameters recorded, 7 fish were assigned to each test and control chamber.
The fish were not fed during the entire study period. Inspection took place after 3, 6, 24, 48, 72 and 96h and involved recording the mortality and visible alterations in appearance and behaviour. Dead fish were removed from the chamber.
Water parameters were measured and recorded before study start and after 0, 24, 48, 72 and 96h.

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

24 h

Dose descriptor:

LC0

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 2

Duration:

24 h

Dose descriptor:

LC50

Effect conc.:

148.3 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 3

Duration:

24 h

Dose descriptor:

LC100

Effect conc.:

220 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 4

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 5

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

148.3 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 6

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

220 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

Concentrations of 0, 10, 22, 50 and 100 ml/L resulted in a mortality of 0 % (0/7) after 24, 48, 72 and 96 h
A concentration of 220 ml/L resulted in a mortality of 100 % (7/7) after 24, 48, 72 and 96 h

Reported statistics and error estimates:

Probit analysis could not be carried out because of the mortality rate. As a factor for the concentration step was 2.2 (third root of 10), no further concentrations were tested. The LC50 is specified as the geometric mean of LC0 and LC100.

Applicant's summary and conclusion

Conclusions:

Under the conditions of this test the LC50 of Tetrakis(piperidino)phosphonium chloride after 96 hours was 148.3 mg/L.