Sodium tetrafluoroborate

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2018

Report date:

2018

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Sodium tetrafluoroborate

EC Number:

237-340-6

EC Name:

Sodium tetrafluoroborate

Cas Number:

13755-29-8

Molecular formula:

NaBF4

IUPAC Name:

sodium tetrafluoroborate

Test material form:

solid

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

During breeding, daphnids were generally fed three times a week with an algal suspension of the green algae Desmodesmus subspicatus supplied by the Collection of Algal Cultures (SAG, Institute for Plant Physiology, University of Göttingen, 37073 Göttingen / Germany) and cultivated at IES Ltd Laboratories under standardized conditions or a mixture of this algal suspension and a commercial fish diet (Tetra Min® Hauptfutter, supplied by TETRA-GmbH, 49324 Melle / Germany).

At the start of the test, the organisms used in the test were 6 24 hours old and were not first brood progeny.

For evaluation of the quality of the daphnia clone and the experimental conditions, potassium dichromate is tested as a positive control twice a year. The result of the latest positive control test in October 2017 (24-hour EC50: 1.3 mg/L, IES Laboratories Study 20170366) showed that the sensitivity of the test organisms was within the range given by the guideline (24-hour EC50: 0.60 2.1 mg/L).

The test method and the test species are recommended by the international test guidelines.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Post exposure observation period:

The immobility of the daphnids was determined by visual inspection after 24 and 48 hours of exposure. Those daphnids not able to swim within 15 seconds after gentle agitation of the test vessel were considered to be immobilized.
Observations were also performed for other non-lethal effects (e.g. abnormal behavior or appearance).

Test conditions

Hardness:

250 mg/L as CaCO3

Test temperature:

The test was performed in a temperature controlled room with continuous monitoring of the room temperature. The test water temperature was maintained between 20 and 21 °C.

pH:

7.8-7.9

Dissolved oxygen:

8.6-8.7 mg/L

Salinity:

0.8

Nominal and measured concentrations:

Nominal Test Item Concentration 1, 10, 100 mg/L

Reference substance (positive control):

yes

Remarks:

potassium dichromate is tested as a positive control twice a year

Results and discussion

Effect concentrations

Key result

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Any other information on results incl. tables

Nominal Test Item Concentration	No. of Daphnids Tested	Immobilized Daphnids after 24 Hours		Immobilized Daphnids after 48 Hours
[mg/L]		No.	[%]	No.	[%]
Control	20	0	0	0	0
100	20	0	0	0	0

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Remarks:

In the control no daphnids showed immobilization or other signs of disease or stress (e.g., discoloration or unusual behavior such as trapping at the surface water).

Conclusions:

The test item Sodium Tetrafluoroborate had no acute toxic effects on Daphnia magna in a static 48-hour test up to the nominal concentration of 100 mg/L under the conditions of the test.