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Diss Factsheets

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Administrative data

Link to relevant study record(s)

Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
12 January to 06 February 2003
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Study undertaken to internationally recognised guideline and GLP.
Justification for type of information:
ANALOGUE APPROACH JUSTIFICATION
Please refer to attached document.

Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
not specified
Qualifier:
according to guideline
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
Deviations:
not specified
GLP compliance:
yes (incl. QA statement)
Analytical monitoring:
yes
Details on sampling:
- Concentrations: 0.1, 0.18, 0.32, 0.56, 1.0, 1.8, 3.2, 5.6 and 10 mg/l
- Sampling method:
- Sample storage conditions before analysis:
Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method:
Four stock solutions were made, CaCl2.2H2O 11.76 g/l; MgSO4.7H2O 4.93 g/l; NaHCO3 2.59 g/l and KCl 0.23 g/l. An aliquot (25 ml) of each of these solutions was added to each litre of deionised water with a conductivity of < 5 µS cm-1 and pH equal to 7.8 +/- 0.2, adjusted if necessary wuth NaOH or HCl. The reconstituted water was aerated until the dissolved oxygen cocncentration was approximately air-saturation value.
Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: Daphnia magna
- Strain:
- Source: In house laboratory cultures
- Age at study initiation (mean and range, SD): < 24 hrs
- Method of breeding: parthenogenesis
- Feeding during test
- Food type: Chlorella sp. (algae)

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Post exposure observation period:
None
Hardness:
250 mg/l CaCO3
Test temperature:
21°C
pH:
7.8 - 7.9
Dissolved oxygen:
8.8 to 8.9 mg O2/l
Nominal and measured concentrations:
Nominal; 0.1, 0.18, 0.32, 0.56, 1.0, 1.8, 3.2, 5.6 and 10 mg/l
Measure; 0.0960, 0.183, 0.337, 0.548, 1.03, 1.87, 3.38, 5.92 and 10.5 mg/l
Details on test conditions:
TEST SYSTEM
- Test vessel:
- Type: closed
- Material, size, headspace, fill volume: 250 ml glass jars with 200 ml of solution
- Aeration: no
- Renewal rate of test solution (frequency/flow rate):
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water:
- Total organic carbon:
- Particulate matter:
- Metals:
- Pesticides:
- Chlorine:
- Alkalinity:
- Ca/mg ratio:
- Conductivity:
- Culture medium different from test medium:
- Intervals of water quality measurement:

OTHER TEST CONDITIONS
- Adjustment of pH: if required
- Photoperiod: 16 hours light, 8 hours dark with 20 minutes dusk.


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mobility at 24 and 48 hours

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 1.8
- Justification for using less concentrations than requested by guideline:
- Range finding study
- Test concentrations: 0.1, 1.0, 10 and 100 mg/l
- Results used to determine the conditions for the definitive study:
Reference substance (positive control):
no
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
5.5 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: 95% CL 4.8 - 6.4 mg/l
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
3.2 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Validity criteria fulfilled:
no
Conclusions:
The 48h EC50 was determined to be 5.5 mg/l and the NOEC 3.2 mg/l

Description of key information

DAP read-across; The 48h EC50 was found to be 5.5 mg/l. The NOEC was 3.2 mg/l.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
5.5 mg/L

Additional information