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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
acute toxicity: oral
Type of information:
experimental study
Adequacy of study:
disregarded due to major methodological deficiencies
Reliability:
3 (not reliable)
Rationale for reliability incl. deficiencies:
unsuitable test system
Remarks:
The study was not designed to test the acute toxic effects of Hydantoin. The study intention was to investigate if pre-treatment with hydantoin is effective with regard to attenuation of DDT-induced neurotoxicity.

Data source

Reference
Reference Type:
publication
Title:
Pharmacological modification of DDT-induced tremor and hyperthermia in rats: distributional factors.
Author:
Herr et al.
Year:
1986
Bibliographic source:
Psychopharmacology (Berl). 1986;89(3):278-83.

Materials and methods

Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
The study was not designed to test the acute toxic effects of Hydantoin. The study intention was to investigate if pre-treatment with hydantoin is effective with regard to attenuation of DDT-induced neurotoxicity.
Test type:
other: alternative test design not aiming to investigate the acute toxic effects of hydantoin

Test material

Constituent 1
Chemical structure
Reference substance name:
Hydantoin
EC Number:
207-313-3
EC Name:
Hydantoin
Cas Number:
461-72-3
Molecular formula:
C3H4N2O2
IUPAC Name:
hydantoin
Test material form:
not specified
Specific details on test material used for the study:
Sigma Chemical Co., St. Louis, MO

Test animals

Species:
rat
Strain:
Fischer 344
Sex:
male
Details on test animals or test system and environmental conditions:
TEST ANIMALS
- Source: Charles River
- Age at study initiation: 9-13 weeks
- Housing: plastic home cages with corncob bedding in groups of four
- Diet: ad libitum
- Water: ad libitum

ENVIRONMENTAL CONDITIONS
- Temperature (°C): 21+/-2 ° C
- Humidity (%): 50+/-10%
- Photoperiod (hrs dark / hrs light): 12 / 12

Administration / exposure

Route of administration:
oral: gavage
Vehicle:
corn oil
Doses:
75 mg/kg
No. of animals per sex per dose:
4
Details on study design:
Two pretreatment groups received hydantoin (75 mg/kg, PO; Sigma Chemical Co., St. Louis, MO) suspended in polyoxyethylene sorbitan mono-oleate (Tween 80; 2drops/10ml distilled water; Sigma Chemical Co., St. Louis, MO)

Results and discussion

Effect levels
Sex:
male
Dose descriptor:
other: NOEL
Effect level:
75 mg/kg bw
Based on:
test mat.
Remarks on result:
not determinable because of methodological limitations

Any other information on results incl. tables

Pharmacological pretreatment had minimal effects on animals not exposed to DDT.

Pretreatment with Hydantoin significantly decreased DDT-induced tremor.

Pharmacological pretreatment also altered DDT-induced hyperthermia. Pretreatment of animals with hydantoin (t=4.86; df=13; P<0.0001) significantly attenuated DDT-induced hyperthermia.

Applicant's summary and conclusion

Interpretation of results:
study cannot be used for classification
Conclusions:
In a study investigating Hydantoin-induced attenuation of DDT-induced neurotoxicity, male rats were pre-treated with 75 mg/kg Hydantoin. US EPA cited an NOEL/LOEL of 75 mg/kg bw. However, as Hydantoin dosing was followed by DDT, a clear NOEL can not be concluded from this study.