2-cyano-N-methylacetamide

Endpoint summary

Description of key information

The substance caused slight reversible effects on rabbit eye and skin (BASF 1981c and d). Procedures were similar to OECD testing guidelines 405and 404. Studies were not performed according to GLP. They are valid with restrictions.

Key value for chemical safety assessment

Skin irritation / corrosion

Endpoint conclusion

Endpoint conclusion:

no adverse effect observed (not irritating)

Eye irritation

Endpoint conclusion

Endpoint conclusion:

no adverse effect observed (not irritating)

Additional information

Skin irritation (Draize test):

The test substance 2 -cyano-N-methylacetamide was investigated for its potential to cause irritation on the rabbit skin according to U.S. Fed.Reg.38, No.187, Para.1500.41, S.27019 (1973) with one single application (BASF 1981c). At least 15 h before application the rabbits (6 animals, 4 males and 2 females) were shaved and the 80 % test solution was applied to intact and scarified skin under occlusion. The test duration was 7 days, and the sacrified animals were pathologically examined, especially in context of necrosis. Only one animal showed at the scarified skin a slight redness 24 h after test substance application. All other investigations were recorded as "doubtful" (intact and scarified skin, redness and edema). Scuffing was observed at one animal after 7 days at the intact as well as at the scarified skin. The Primary Irritation Index was calculated according to the scheme of Draize (1965), resulting in a value of 1.0, indicating no skin irritation potential of the test substance.

Eye irritation (Draize test, similar to OECD 405):

The test substance was also investigated for its potential to cause eye irritation (in vivo-test with rabbit, according to U.S. Fed.Reg.38, No.187, Para.1500.42, S.27019 (1973) with one single application (BASF 1981d). About 50 mg of crushed substance (bulk volume of 0.1 mL) was applied into the conjunctival sac and not washed out afterwards. The untreated eye served as negative control. 6 animals (2 males and 4 females) were observed for 10 days after this procedure. Ciliary injection as well as a slight opacity of the cornea (three quarters of the area) and significant redness, slight swelling and secretion of the conjunctiva was recorded 24 h after test substance application. In one animal contraction of the pupils was observed after 24 h and 48 h. At day 10 no symptons were recorded. The Primary Irritation Index according to the scheme of Draize (1965) amounts to 16, which corresponds to a slightly irritating test substance for the rabbit eye mucosa.

Effect level: empty Endpoint conclusion: Adverse effect observed

Justification for classification or non-classification

Dangerous Substance Directive (67/548/EEC)

The available studies are considered reliable and suitable for classification purposes under 67/548/EEC. As a result the substance is not considered to be classified for skin or eye irritation under Directive 67/548/EEC, as amended for the 28th time in Directive 2001/59/EC.

Classification, Labelling, and Packaging Regulation (EC) No. 1272/2008

The available experimental test data are reliable and suitable for classification purposes under Regulation 1272/2008. Slight reversible effects occurred, but means for 24, 48 and 72h for iris, cornea and conjunctivea were below the thresholds for classification as an irritant. As a result the substance is not considered to be classified for skin or eye irritation under Regulation (EC) No. 1272/2008, as amended for the second time in Directive EC 286/2011.