2,3-epoxybutane

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: test procedure in accordance with German national standards

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1989

Report date:

1988

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: DIN 38412, Part 9, cell multiplication inhibitory test

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

1,2-epoxybutane

EC Number:

203-438-2

EC Name:

1,2-epoxybutane

Cas Number:

106-88-7

Molecular formula:

C4H8O

IUPAC Name:

2-ethyloxirane

Details on test material:

CHEMICAL NAME: 1,2-epoxybutane

Sampling and analysis

Analytical monitoring:

no

Test organisms

Test organisms (species):

Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)

Details on test organisms:

- Scenedesmus subspicatus CHODAT SAG 86.81
- Supplier: SAG (Collection of algal cultures in Goettingen, GER)

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

72 h

Test conditions

Test temperature:

23°C

Nominal and measured concentrations:

- nominal test concentrations: 3.9, 7.8, 15.63, 31.25, 62.5, 125, 250 and 500 mg/L

Details on test conditions:

TEST CONDITIONS
- dilution water chemistry: according to OECD guideline 201
- exposure vessels: volume 10 mL
- number of Replicates: 4
- initial cell number: 10000 cells/mL

MEASUREMENTS
- in vivo chlorophyll-a-fluorescence (685 nm) after 0, 24, 48 and 72 hours

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

> 500 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Effect concentrations 2

Duration:

72 h

Dose descriptor:

EC90

Effect conc.:

> 500 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Effect concentrations 3

Duration:

72 h

Dose descriptor:

EC20

Effect conc.:

130 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Any other information on results incl. tables

GROWTH RATE ACCORDING TO THE CONTROL [%]
Concentration	24h	48h	72h
  3.91 mg/L	114	106	 97.2
  7.81 mg/L	116	115	104
 15.63 mg/L	108	117	104
 31.25 mg/L	 91.9	 99.1	 98.9	
 62.5  mg/L	 87.7	 91.9	 92.7	
125    mg/L	 75.7	 82.7	 80.5	
250    mg/L	 66.9	 71.8	 67.0	 
500    mg/L	 57.3	 52.8	 51.1	 

EC-VALUES [mg/L]
24h-EC20  =  100
24h-EC50  = >500
24h-EC100 = >500

48h-EC20  =  160
48h-EC50  = >500
48h-EC100 = >500

72h-EC20  =  130
72h-EC50  = >500
72h-EC100 = >500