Reaction mass of pentasodium 2-{[4-chloro-6-(ethyl{3-[(2-sulfonatoethyl)sulfonyl]phenyl}amino)-1,3,5-triazin-2-yl]amino}-5-hydroxy-6-({2-sulfonato-4-[(4-sulfonatophenyl)diazenyl]phenyl}diazenyl)naphthalene-1,7-disulfonate and tetrasodium 2-[(4-chloro-6-{ethyl[3-(vinylsulfonyl)phenyl]amino}-1,3,5-triazin-2-yl)amino]-5-hydroxy-6-({2-sulfonato-4-[(4-sulfonatophenyl)diazenyl]phenyl}diazenyl)naphthalene-1,7-disulfonate

Adsorption / desorption

Link to relevant study record(s)

Reference

Endpoint:

adsorption / desorption: screening

Type of information:

experimental study

Adequacy of study:

key study

Study period:

10 April 2006 to 19 May 2006

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 121 (Estimation of the Adsorption Coefficient (Koc) on Soil and on Sewage Sludge using High Performance Liquid Chromatography (HPLC))

Deviations:

yes

Remarks:

See overall remarks, attachment

Qualifier:

according to guideline

Guideline:

EU Method C.19 (Estimation of the Adsorption Coefficient (KOC) on Soil and Sewage Sludge Using High Performance Liquid Chromatography (HPLC))

GLP compliance:

yes (incl. QA statement)

Type of method:

HPLC estimation method

Media:

soil

Specific details on test material used for the study:

Identity: FAT 40824/A
Batch: Red ROE 805 BOP 04/05
Appearance: dark red powder
Purity: Organic part (Na-salt): approx. 82 %; Main component 1 : approx. 36.2 %; Main component 2: approx. 27.5 %; Oligomers: 10%
Expiration date: 01 October 2010
Storage: At room temperature at about 20°C in a desiccator (hygroscopic)

Radiolabelling:

no

Test temperature:

25 °C

Details on study design: HPLC method:

This method utilises a high performance liquid chromatograph with UV/VIS detector, reversed phase column and an aqueous mobile phase.
HPLC-Conditions:
Apparatus: Varian autosampler ProStar 410
Varian Pump ProStar 230
Varian UV/VIS detector ProStar 310
Jones column oven 7990
Column: LiChrospher 100 CN, particle size 5 μm, 250 mm x 4 mm
Pre-column: CN
Mobile phase: Water / methanol (45:55; v/v); pH 6.3
Detection: Test item: 254 nm
Reference items: 210 nm
Sodium nitrate: 210 nm
Temperature: 25 °C
Injection volume: 20 μl
Flow: 1.0 ml/min
Run time: 15 minutes

Details on sampling:

Not applicable

Details on matrix:

Not applicable

Details on test conditions:

Not applicable

Computational methods:

Not applicable

Type:

log Koc

Value:

< 1.25 other: not specified

Temp.:

25 °C

Type:

Koc

Value:

< 18 other: not specified

Temp.:

25 °C

Details on results (HPLC method):

The test item was not retained under the chromatographic conditions of the test and was eluted at the dead time.
log Koc < 1.25
Koc < 18

Adsorption and desorption constants:

Not applicable

Recovery of test material:

Not applicable

Concentration of test substance at end of adsorption equilibration period:

Not applicable

Concentration of test substance at end of desorption equilibration period:

Not applicable

Transformation products:

not specified

Details on results (Batch equilibrium method):

Not applicable

Statistics:

Not applicable

Validity criteria fulfilled:

yes

Conclusions:

In conclusion, the log of the adsorption coefficient (KOC) of test item was estimated to be log KOC < 1.25 which is equal to a KOC value of < 18 using the HPLC method. This value indicates that the test item will not be adsorbed by organic carbon in soil. The test item can be considered to be of very high mobility.

Executive summary:

In a GLP compliant study, the determination of the adsorption coefficient (K_OC) of test item was performed according to the OECD Guideline for the Testing of Chemicals, No. 121, Estimation of the Adsorption Coefficient (K_OC) on Soil and Sewage Sludge using High Performance Liquid Chromatography (HPLC), January, 2001 and the EC Directive 2001/59, C.19.

The test item was not retained under the chromatographic conditions of the test and was eluted at the dead time. The K_OCof test item was therefore determined to be lower than that one of the lowest reference item used (acetanilide). The chromatography of the test item resulted in one main peak with a well-defined and reproducible retention time. The HPLC method was therefore considered to be suitable for the purpose of the study. Based on the chromatographic data, the test item was considered to be stable during the test procedure. In conclusion, the log of the adsorption coefficient (K_OC) of test item was estimated to be log K_OC <1.25 which is equal to a K_OC value of < 18 using the HPLC method. This value indicates that the test item will not be adsorbed by organic carbon in soil. The test item can be classified to be of very high mobility.

Description of key information

The KOC of the test substance is <18 and the logKOC accordingly <1.25.

Key value for chemical safety assessment

Koc at 20 °C:

18

Additional information

In a GLP compliant study, the determination of the adsorption coefficient (K_OC) of test item was performed according to the OECD Guideline for the Testing of Chemicals, No. 121, Estimation of the Adsorption Coefficient (K_OC) on Soil and Sewage Sludge using High Performance Liquid Chromatography (HPLC), January, 2001 and the EC Directive 2001/59, C.19.

The test item was not retained under the chromatographic conditions of the test and was eluted at the dead time. The K_OC of test item was therefore determined to be lower than that one of the lowest reference item used (acetanilide). The chromatography of the test item resulted in one main peak with a well-defined and reproducible retention time. The HPLC method was therefore considered to be suitable for the purpose of the study. Based on the chromatographic data, the test item was considered to be stable during the test procedure. In conclusion, the log of the adsorption coefficient (K_OC) of test item was estimated to be log K_OC <1.25 which is equal to a K_OC value of < 18 using the HPLC method. This value indicates that the test item will not be adsorbed by organic carbon in soil. The test item can be classified to be of very high mobility.

[LogKoc: 1.25]