2,4-dinitrophenol

Genetic toxicity: in vivo

Administrative data

Endpoint:

in vivo mammalian germ cell study: cytogenicity / chromosome aberration

Remarks:

Type of genotoxicity: chromosome aberration

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Data have been obtained from secondary source.

Data source

Reference

Reference 1

Reference Type:

secondary source

Title:

Unnamed

Year:

1995

Reference 2

Reference Type:

review article or handbook

Title:

Effect of some phenolic compounds on chromosomes of bone marrow cells in mice

Author:

Mitra AB, Manna GK.

Year:

1971

Bibliographic source:

Indian J Med Res 59:1442-1447.

Materials and methods

Principles of method if other than guideline:

The method is based on a chromosome aberration assay in vivo. An intraperitoneal injection of three saturated solution concentrations was made on mice. After 24 hours bone marrow cells were analyzed. No further details were specified.

GLP compliance:

no

Type of assay:

chromosome aberration assay

Test material

Test material information

Constituent 1

Reference substance name:

2,4-dinitrophenol

EC Number:

200-087-7

EC Name:

2,4-dinitrophenol

Cas Number:

51-28-5

Molecular formula:

C6H4N2O5

IUPAC Name:

2,4-dinitrophenol

Test material form:

other: saturated solution

Test animals

Species:

mouse

Strain:

not specified

Sex:

not specified

Administration / exposure

Route of administration:

intraperitoneal

Post exposure period:

Mice were sacrified 24 hours posttreatment for analysis of bone marrow cells.

Doses / concentrations

Doses / concentrations 1

Remarks:

Doses / Concentrations:
0.25 ml
Basis:
other: saturated solution

Doses / concentrations 2

Remarks:

Doses / Concentrations:
0.5 ml
Basis:
other: saturated solution

Doses / concentrations 3

Remarks:

Doses / Concentrations:
1 ml
Basis:
other: saturated solution

Examinations

Tissues and cell types examined:

Bone marrow cells was observed for chromosomal aberration analysis.

Results and discussion

Test results

Sex:

not specified

Genotoxicity:

positive

Toxicity:

not specified

Any other information on results incl. tables

The authors concluded that 2,4-DNP was clastogenic under the assay conditions and attributed the effect to the compound’s electrophilic properties.

However there was no linear relationship between the frequency of chromosome aberrations and the dose of 2,4-dinitrophenol.

Applicant's summary and conclusion

Conclusions:

Interpretation of results (migrated information): positive
2,4-DNP was clastogenic under the assay conditions due to compound’s electrophilic properties. However there was no linear relationship between the frequency of chromosome aberrations and the dose of 2,4-dinitrophenol.

Executive summary:

Data have been obtained from secondary source that mentions Mitra AB, Manna GK. 1971. Effect of some phenolic compounds on chromosomes of bone marrow cells in mice. Indian J Med Res 59:1442-1447.

The method is based on a chromosome aberration assay in vivo. Mice were injected intraperitoneally with 0.25, 0.50, and 1 mL of a saturated solution of 2,4-DNP, then sacrificed 24 hours posttreatment for analysis of bone marrow cells for chromosomal aberrations . No further details were specified.

The authors concluded that 2,4-DNP was clastogenic under the assay conditions and attributed the effect to the compound’s electrophilic properties.

However there was no linear relationship between the frequency of chromosome aberrations and the dose of 2,4-dinitrophenol.