Alcohols, C12-18, reaction products with D-glucose

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

14.12.10 – 24.02.11

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: study performed following OECD 301 F and the lab is certified GLP

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2010

Report date:

2011

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

LCE10077

IUPAC Name:

LCE10077

Details on test material:

Product name: LCE 10077
Product code: 07/00012; Lot: T01745
Form: solid
Origin: Safety Data Sheet of 01.02.07
purity:100%
substance type: UVCB

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material (migrated information):
PHYSICO-CHEMICAL PROPERTIES
- Melting point:
- Boiling point:
- Vapour pressure:
- Henry's law constant (for volatie substances):
- Water solubility (under test conditions):
- Solubility in organic solvents:
- log Pow:
- Stability of test material at room temperature:stable


OTHER PROPERTIES (if relevant for this endpoint)
- Toxicity to microorganisms:no

PHYSICO-CHEMICAL PROPERTIES
- Vapour pressure:extrapolated at 25 °C is 1.7 x 10-3 Pa.
- Water solubility (under test conditions):The test item is a solid soluble in water and was put directly into the test ves-sels.
- log Pow:Therefore, the log P of LCE10027 is equal to 7.7.


OTHER PROPERTIES (if relevant for this endpoint)
- Adsorption characteristics:adsorption/desorption coefficient on soil and on sewage sludge can be
estimated to be higher then 2.4.
- Toxicity to microorganisms: none
- Other:

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge (adaptation not specified)

Details on inoculum:

Activated sludge from the municipal sewage treatment plant Reutlingen-West was washed two times with tap water and filtered (Liquid jet vacuum pump with Filter paper circles, Schleicher & Schuell); 1 g of the wet filtrate corresponded to approximately 60 mg suspended solids. Using a test volume of 250 mL, 100 mg of the wet filtrate were inserted per flask, corresponding to 24 mg sus-pended solids per litre.

Duration of test (contact time):

28 d

Initial test substance concentration

Initial conc.:

100 mg/L

Based on:

act. ingr.

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

other: biological oxygen demand: BOD

Details on study design:

- Dilution water: Deionised water was aerated for 2 h with a membrane pump and allowed to stand for 0.5 h before usage. This water was used for preparation of the mineral medium for the blanks and as dilution water for the test item and reference item.
- Mineral medium: The following stock solutions were prepared:
solution 1: KH2PO4 8.50 g/L
K2HPO4 21.75 g/L
Na2HPO4 x 2 H2O 33.40 g/L
NH4Cl 0.50 g/L
solution 2: CaCl2 x 2 H2O 27.50 g/L
solution 3: MgSO4 x 7 H2O 22.50 g/L
solution 4: FeCl3 x 6 H2O 0.25 g/L
The stock solutions were diluted in deionised water for preparation of the Min-eral medium for test flasks 1 – 6 directly before the start of the test. 1 L of the Mineral medium included 10 mL of solution 1 and 1 mL of solutions 2 – 4.


- Apparatus: BOD-digi (SELUTEC GmbH), electrolytic oxygen supply with automatic data registration and temperature regulation.
- Incubation: 28 days, stirred culture at 22 ± 2 °C, incubator.
- Test item: The test item is a solid soluble in water and was put directly into the test ves-sels.
- Reference
item: Sodium-Benzoate in Mineral medium.

Flasks: 1: Test item in Mineral medium + inoculum
2: Test item in Mineral medium + inoculum
3: Mineral medium + inoculum (blank)
4: Mineral medium + inoculum (blank)
5: Reference item Sodium-Benzoate in Mineral medium + inoculum
6: Test item in Mineral medium, sterilised with HgCl2/L
(C HgCl2 = 10 g/L; abiotic control)

Reference substance

Reference substance:

benzoic acid, sodium salt

Results and discussion

% Degradation

% Degradation 1

Parameter:

other: ThOD

Value:

ca. 49.4

Sampling time:

14 d

% Degradation 2

Parameter:

other: ThOD

Value:

ca. 41.2

Sampling time:

10 d

% Degradation 3

Parameter:

other: ThOD

Value:

ca. 84

Sampling time:

28 d

Details on results:

The validity criteria of OECD-Guideline 301 F are accomplished:
- Maximum BOD-difference in flasks 1 + 2 at the end of the test: 0.7 % (nominal value: < 20 %)
- Mean value of inoculum blank: 23.6 mg O2/L (nominal value:  60 mg O2/L)
- Biodegradation of reference item after 14 d: 89.7 % (nominal value: > 60 %)

BOD5 / COD results

BOD5 / COD

Parameter:

BOD5

Value:

ca. 2.29 g O2/g test mat.

Results with reference substance:

Reference item:
- Test concentration: 100.4 mg/L
- ThOD of the reference item: 1.67 mg O2/mg reference item
- ThOD in flask 5: 167.7 mg O2/L
- BOD in flask 5 after 28 days: 184.3 mg O2/L (including blank)
- BODreference item - BODinoculum blank: 160.7 mg O2/L
or: 1.60 mg O2/mg reference item
- Biodegradation after 14 days: 89.7 %
- Biodegradation after 28 days: 95.8 %
- 10 days window: 87.3 %
The pH in flask 5 originally was 7.7 and was corrected to 7.5 by addition of 1 N HCl at the beginning of the test. At the end of the test the pH in flask 5 was 7.8.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

readily biodegradable, but failing 10-day window

Conclusions:

The test item LCE 10077 achieved 84.0 % biodegradation in 28 days (10 days window: 41.2 %) in a Manometric Respirometry Test conducted according to OECD-Guideline 301 F.
The test item LCE 10077 is readily biodegradable not meeting the 10 days window.

Executive summary:

The test item LCE 10077 achieved 84.0 % biodegradation in 28 days (10 days window: 41.2 %) in a Manometric Respirometry Test conducted according to OECD-Guideline 301 F. The test item LCE 10077 is readily biodegradable not meeting the 10 days window.