Perhydropyrimidin-2-one

Specific investigations: other studies

Administrative data

Endpoint:

biochemical or cellular interactions

Type of information:

experimental study

Adequacy of study:

other information

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Meets generally accepted scientific standards, well documented and acceptable for assessment

Data source

Reference

Reference Type:

publication

Title:

Cyclic Urea and Thiourea Derivatives as Inducers of Murine Erythroleukemia Differentiation

Author:

Li, C.-d. et al.

Year:

1981

Bibliographic source:

J. Med. Chem. 24, 1089-1092

Materials and methods

Principles of method if other than guideline:

Cyclic urea and thiourea derivatives were compared to Me2SO and to tetramethylurea as inducers of the differentiation of murine erythroleukemia cells; concurrently, their effects on cellular replication were measured. These measurements were accomplished by exposing exponentially growing murine erythroleukemia cells to a wide range of concentrations of each agent to determine the optimal level required for maximum differentiation. The degree of erythroid maturation was measured by assessing the proportion of benzidine-positive cells (i .e., hemoglobincontaining cells) 6 days after continuous exposure to the agent under test. Cell numbers were determined on days 3 and 6 of incubation.

GLP compliance:

no

Type of method:

in vitro

Endpoint addressed:

other: cell replication and differentiation

Test material

Test material information

Constituent 1

Reference substance name:

Propyleneurea

IUPAC Name:

Propyleneurea

Administration / exposure

Doses / concentrations

Remarks:

Doses / Concentrations:
4 mM
Basis:
nominal conc.

Results and discussion

Details on results:

Propyleneurea did not inhibit cell growth. 15% of the cells were benzidine-positive after incubation with propyleneurea, indicating that propyleneurea was a relatively weak inducer of differentiation in this assay.

Any other information on results incl. tables

Effects of Propyleneurea on the Replication and Erythroid Differetiation of Friend Leukemia Cells:

Test substance	Concentration, mM	% cell growth on day 3^a	% cell growth on day 6^a	benzidine-positive cells, %
Propyleneurea	4	124	121	15
Me2SO^b	105	70	120	75
Me2SO^b	210	72	110	94

^a Percent of untreated control cell growth.^b Me₂SO was included as a control in all experiments.

Applicant's summary and conclusion

Conclusions:

Propyleneurea did not inhibit cell growth and was a relatively weak inducer of differentiation in this assay using Friend Murine Erythrolekemia cells.