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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Administrative data

Link to relevant study record(s)

Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Version / remarks:
2004
Qualifier:
according to guideline
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
GLP compliance:
yes (incl. QA statement)
Test organisms (species):
Daphnia magna
Test type:
static
Water media type:
freshwater
Total exposure duration:
48 h
Hardness:
The total hardness and alkalinity of the batch of Elendt M4 medium used were 262 and 83 mg/L as CaCO3, respectively.
Test temperature:
20.9 (see details in table under "Any other information on materials and methods" below)
pH:
8.2 (see details in table under "Any other information on materials and methods" below)
Dissolved oxygen:
99.7 (see details in table under "Any other information on materials and methods" below)
Nominal and measured concentrations:
Nominal concentrations of 4.27, 9.39, 20.7, 45.5 and 100 mg/L.
Details on test conditions:
OTHER TEST CONDITIONS
- Adjustment of pH:
In the preliminary test, the pH of the test medium at 100 mg/L was above the maximum (pH 9) recommended for this type of test. Therefore in the definitive test, the media at 20.7, 45.5 100 mg/L were prepared with pH adjustment (range 6 – 9) and without pH adjustment.


RANGE-FINDING STUDY
- Test concentrations:
- Results used to determine the conditions for the definitive study:
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Basis for effect:
mobility
Remarks on result:
other:
Remarks:
pH adjusted
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
45.5 mg/L
Nominal / measured:
nominal
Basis for effect:
mobility
Remarks on result:
other:
Remarks:
pH adjusted
Validity criteria fulfilled:
yes
Conclusions:
The 48-hour EC50 of APD for the immobilisation of Daphnia magna was 52.5 mg/L when performed without pH adjustment and > 100 mg/L following pH adjustment of the media.
The ‘no-observed effect concentration’ (NOEC) was 20.7 mg/L without pH adjustment and 45.5 with pH adjustment.
These results suggested that the pH of the medium was primarily responsible for the immobilisation observed.

Description of key information

Based on OECD 202, the 48 -hour EC50 of APD for the immobilisation of Daphnia magna is > 100 mg/L following pH adjustment of the media. The ‘no-observed effect concentration’ (NOEC) is 45.5 mg/L with pH adjustment.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
100 mg/L

Additional information