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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Remarks:
Results are based on calculated concentration data which are gained out of a non-validated HPLC method
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study with acceptable restrictions

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2022
Report date:
2022

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
Ethyl 4-(bromomethyl)-2-[(2,6-difluorophenyl)methyl-ethoxycarbonylamino]-5-(4-nitrophenyl)thiophene-3-carboxylate
Cas Number:
308831-95-0
Molecular formula:
C24H21BrF2N2O6S
IUPAC Name:
Ethyl 4-(bromomethyl)-2-[(2,6-difluorophenyl)methyl-ethoxycarbonylamino]-5-(4-nitrophenyl)thiophene-3-carboxylate
Test material form:
solid: particulate/powder

Sampling and analysis

Analytical monitoring:
no
Remarks:
Results are based on calculated concentration data which are gained out of a non-validated HPLC method

Test solutions

Vehicle:
yes
Remarks:
Elendt M4 medium

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
Test species: Daphnia magna Straus
Age: < 24 hours neonates
Origin: National Institute of Environmental Health

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h

Test conditions

Test temperature:
21 ± 1 °C
pH:
7.181 - 7.292 (At the start (d0))
6.869 - 6.919 (After 48h (d2))
Dissolved oxygen:
6.16 - 7.99 (At the start (d0))
8.17 - 8.57 (After 48h (d2))
Nominal and measured concentrations:
0.000018; 0.000037; 0.000055; 0.000073 and 0.00011 mg/L (nominal concentration)
Reference substance (positive control):
not required

Results and discussion

Effect concentrationsopen allclose all
Duration:
48 h
Dose descriptor:
EC10
Remarks on result:
not determinable
Duration:
48 h
Dose descriptor:
EC50
Remarks on result:
not determinable
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
>= 0 mg/L
Nominal / measured:
nominal
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
LOEC
Effect conc.:
> 0 mg/L
Nominal / measured:
nominal
Basis for effect:
mobility

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
According to the test results BNTC has no toxic effect on Daphnia immobilisation even at its saturated concentration (0.00011 mg/L).