cis-4-[3-(p-tert-butylphenyl)-2-methylpropyl]-2,6-dimethylmorpholine

Phototransformation in water

Administrative data

Endpoint:

phototransformation in water

Type of information:

experimental study

Adequacy of study:

other information

Study period:

April 1987- September 1988

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1988

Report date:

1988

Materials and methods

Study type:

direct photolysis

Test guideline

Qualifier:

according to guideline

Guideline:

EPA Guideline Subdivision N 161-2 (Photodegradation Studies in Water)

Version / remarks:

June 1985

GLP compliance:

yes

Remarks:

study was conducted prior to the implementation of GLP certificates

Test material

Test material information

Constituent 1

Reference substance name:

cis-4-[3-(p-tert-butylphenyl)-2-methylpropyl]-2,6-dimethylmorpholine

EC Number:

266-719-9

EC Name:

cis-4-[3-(p-tert-butylphenyl)-2-methylpropyl]-2,6-dimethylmorpholine

Cas Number:

67564-91-4

Molecular formula:

C20H33NO

IUPAC Name:

(2R,6S)-4-[3-(4-tert-butylphenyl)-2-methylpropyl]-2,6-dimethylmorpholine

Details on test material:

IUCLID4 Test substance: other TS: 14-C Fenpropimorph

Study design

Radiolabelling:

yes

Remarks:

phenyl-U-14C-labelled fenpropimorph

Analytical method:

gas chromatography

mass spectrometry

other: thin layer chromatography

Buffers:

1 mM sodium acetate buffer

Light source:

Xenon lamp

Relative light intensity:

100 000

Details on test conditions:

The direct photolysis was performed with phenyl-U-14C-labelled fenpropimorph. The specific radioactivity was 166.69 MBq/g (purity >98%). The study was performed at pH 5 (sodium acetate buffer). The active substance is hydrolytically stable at this pH-value. The concentration of the active substance in the sterile aqueous buffer solution was about 9 mg/l. Each 31.5 ml of the sterile solution was transferred to sterile open shallow glass dishes. The boxes were placed under a xenon lamp and sealed with quartz glass covers. The light intensity of the simulated sunlight was about 100.000 lx. The study was carried out for 30 days at 25 +/- 2°C with day and night cycle. Additionally, a dark control was investigated. Since the active substance turned out to be stable upon irradiation, 2% (related to the final volume of the respective solution) of a photosensitising reagent (acetone) were added to further samples and the irradiation procedure was repeated. Samples (content of one glass dish) were taken after 0, 1, 2, 4, 8, 16/15 (samples without/with photosensitising reagent), and 30 days. All samples were irradiated in duplicate to confirm reproducibility.

Duration of test at given test condition

Duration:

30 d

Temp.:

25 °C

Initial conc. measured:

10 other: mg a.i./kg

Dark controls:

yes

Results and discussion

% Degradation

% Degradation 1

Test condition:

Direct photolysis

% Degradation 2

Test condition:

Indirect photolysis

Details on results:

The results of the tests with and without acetone are compared in Table 7.2/2. Based on TLC analysis and MS identification it was proved that no degradation of the active substance occured. Minor amounts of BF 421-13 (identified by MS analysis) as well as of other unidentified degradation products could be detected. Their amounts did not exceed 10% TAR. However, these impurities were already detected in comparable quantities in the stock solution of the active substance and therefore were no photodegradation products.

Any other information on results incl. tables

Table 7.2/2     Recovery of radioactivity in % after application of phenyl-U-^14C-labelled fenpropimorph during aqueous photolysis
Irradiation time	Recovery (% TAR)
(d)	Samples without acetone	Samples with acetone
0	n.d.	n.d.
1	100.8	101.1
2	107.1	94.0
4	100.8	97.9
8	91.5	96.6
15/16 *	91.0	91.6
30	85.4	91.6
30, dark	112.7	104.6

*:          without/with acetone

n.d.:     not determined

 

Applicant's summary and conclusion

Conclusions:

It can be stated that fenpropimorph is photolytically stable. No photolytical half-life of fenpropimorph was calculated.