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EC number: 250-466-6 | CAS number: 31098-21-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 25 January - 31 January 1983
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: GLP - guideline study
Data source
Referenceopen allclose all
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 983
- Report date:
- 1983
- Reference Type:
- other: Amendment to study report project 015041
- Title:
- Unnamed
- Year:
- 2 004
- Report date:
- 2004
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- other: OECD Guideline Draft No. 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- yes
- Remarks:
- (strains: Salmonella typhimurium TA1535, TA 1537, TA 98, TA100 and TA 1538 were used)
- Qualifier:
- according to guideline
- Guideline:
- other: EU Method B.14 (Draft) , (Mutagenicity - Reverse Mutation Test Using Bacteria)
- Deviations:
- yes
- Remarks:
- (strains: Salmonella typhimurium TA1535, TA 1537, TA 98, TA100 and TA 1538 were used)
- GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Potassium 3-sulphonatopropyl acrylate
- EC Number:
- 250-465-0
- EC Name:
- Potassium 3-sulphonatopropyl acrylate
- Cas Number:
- 31098-20-1
- Molecular formula:
- C6H10O5S.K
- IUPAC Name:
- potassium 3-(acryloyloxy)propane-1-sulfonate
- Details on test material:
- - Name of test material (as cited in study report): 3-Sulfopropyl-methacrylsauereester , SPM
- Substance type: white crystal
- Analytical purity: 98%
- Lot/batch No.: 31098-21-2
- Storage condition of test material: at room temperature , in the dark
- Other: To avoid any light effects on the test compound, all experimentation was performed under yellow light.
Constituent 1
Method
- Target gene:
- his-
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Species / strain / cell type:
- S. typhimurium TA 1538
- Metabolic activation:
- with and without
- Metabolic activation system:
- Liver microsomal fraction (S9 mix), prepared from the livers of male Wistar rats treated with Aroclor 1254
- Test concentrations with justification for top dose:
- 1.58, 5, 15.8, 50, 158, 500, 1580 and 5000 µg/plate with and without metabolic activation
- Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: distilled water
- On the day of the experiment, the test material was dissolved in distilled water at the highest investigated dose. The other doses were dilutions from this stock solution with the solvent in half-log intervals.
Controls
- Untreated negative controls:
- other: solvent control (distilled water)
- Negative solvent / vehicle controls:
- yes
- Remarks:
- distilled water
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- 9-aminoacridine
- 2-nitrofluorene
- N-ethyl-N-nitro-N-nitrosoguanidine
- benzo(a)pyrene
- methylmethanesulfonate
- other: 2-aminoanthracene
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: in agar (plate incorporation)
DURATION
- Preincubation period: The bacteria were grown overnight in a shaking water bath for 16 hours at 37°C in 2.5% Nutrient Broth No. 2. After centrifugation, the bacteria were resuspended to a concentration of approximately 1 x 10E8 to 2 x 10E9 cells per millilitre in 0.16% Nutrient Broth and 0.5% Sodium chloride. The concentration of germs was controlled photometrically and determined in an experimental test with Histidine-rich potassium chloride solution on selective agar plates.
DETERMINATION OF CYTOTOXICITY
- To estimate the toxicity of the test material prototrophic bacteria (spontaneous revertants of TA 1537 = RTA) were added as an internal standard of to the selective agar plates together with TA 1537. The difference in the number of colonies on plates with and without added prototrophic bacteria at each dose level of the test material was compared to the number of colonies obtained with the negative control. The ratio of the two values was expressed as relative survival rate with the negative control at a 100 % survival rate.
Additionally, the toxicity of the test material can be evidenced in the mutagenicity assay, by a reduction in the number of revertants or by a clearing of the background lawn. - Evaluation criteria:
- A material is identified as a mutagen in this test system if there is a reproducible demonstration of a dose effect relation with a 2-fold increase in the number of revertants over the controls in at least one strain . With the strain TA 100 a 1.5-fold increase is the criterion for a positive result .
- Statistics:
- The difference in the number of colonies on plates with and without added prototrophic bacteria at each dose level of the test material was compared to the number of colonies obtained with the negative control. The ratio of the two values was expressed as relative survival rate with the negative control at a 100 % survival rate.
Additionally, the toxicity of the test material can be evidenced in the mutagenicity assay, by a reduction in the number of revertants or by a clearing of the background lawn.
Results and discussion
Test resultsopen allclose all
- Key result
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Remarks:
- (No relevant increase of the revertant colony numbers was obtained in Salmonella typhimurium strain used at all dose levels tested in comparison with the corresponding controls. The presence of microsomal activation did not influence these findings.)
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity nor precipitates, but tested up to recommended limit concentrations
- Remarks:
- (In the toxicity experiment with 3-Sulfopropyl-methacrylsauereester (SPM), neither quantitative nor qualitative evidence of a toxic effect was observed.)
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 1538
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Remarks:
- (No relevant increase of the revertant colony numbers was obtained in Salmonella typhimurium strain used at all dose levels tested in comparison with the corresponding controls. The presence of microsomal activation did not influence these findings.)
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Remarks:
- (In the toxicity experiment with 3-Sulfopropyl-methacrylsauereester (SPM), neither quantitative nor qualitative evidence of a toxic effect was observed.)
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Additional information on results:
- In the current study, the following results have been compiled:
GERM CONCENTRATIONS :
The concentrations of Salmonella typhimurium used in this assay were as follows:
TA 98 = 9.8 x 10 exp. 8 cells/ml
TA 100 = 5.7 x 10 exp. 8 cells/ml
TA 1535 = 1.5 x 10 exp. 9 cells/ml
TA 1537 = 9.9 x 10 exp. 8 cells/ml
TA 1530 = 1.3 x 10 exp. 9 cells/ml
in the experiment without metabolic activation,
TA 98 = 1.5 x 10 exp. 9 cells/ml
TA 100 = 7.4 x 10 exp. 8 cells/ml
TA 1535 = 1.3 x 10 exp. 9 cells/ml
TA 1537 = 1.5 x 10 exp. 9 cells/ml
TA 1538 = 1.3 x 10 exp. 9 cells/ml
in the experiment with metabolic activation.
Control plates with the used solvent (negative control) showed numbers of spontaneous revertant colonies per plate which were within the normal range of those cited in the literature .
Control plates with reference mutagens (positive controls) showed a distinct increase of the revertant colonies with the tester strains. This confirmes the reversion properties of each strain. The positive results of the mutagens 2-Amino-anthracene and Benzo(a)pyrene indicate that the metabolizing system was functioning.
The aseptic control showed no contamination for the S-9 mix. - Remarks on result:
- other: all strains/cell types tested
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results:
negative with and without metabolic activation
The study was performed according to the OECD Guideline 471 with deviations (strain TA 1538 instead of TA 102) according to the principles of the good laboratory practice and therefore considered to be of high quality (reliability Klimisch 2). The vehicle and the positive control substances fulfilled validity criteria of the test system. The test material did not induce significant increases in the frequency of revertant colonies in any of the bacterial strains. The test material was considered to be non-mutagenic under the conditions of the test. - Executive summary:
The test substance 3-Sulfopropyl-methacrylsauereester (SPM) was investigated according to OECD TG471 for its potential to cause gene mutations in Salmonella typhimurium strains (TA98, TA100, TA1537, TA1535 and TA1538). Under the conditions of this experiment 3-Sulfopropyl-methacrylsauereester (SPM) was found to cause no toxic effects. Up to the highest investigated dose, no relevant increase of the revertant colony numbers was obtained in any Salmonella typhimurium strain in comparison with the corresponding controls. The presence of microsomal activation did not influence these findings. In conclusion, it can be stated that during the described mutagenicity test and under the experimental conditions reported, the test material caused neither base-pair substitutions, nor frameshift mutations. Therefore the test results with 3-Sulfopropyl-methacrylsauereester (SPM) revealed no indication of gene mutagenic activity.
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