Ammonium hexafluorosilicate

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2011/05/26 - 2011/06/19

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2011

Report date:

2011

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Version / remarks:

2014/04/13

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

1

Reference substance name:

Ammonium hexafluorosilicate

EC Number:

240-968-3

EC Name:

Ammonium hexafluorosilicate

Cas Number:

16919-19-0

Molecular formula:

F6Si.2H4N

IUPAC Name:

diammonium hexafluorosilicate

Test material form:

solid: particulate/powder

Specific details on test material used for the study:

Ammoniumhexafluorosilicate; Batch No.: GRA 2011-007; purity: 98.9 %.

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

Duplicate samples from the freshly prepared test medium of the only test concentration and the control were taken at the start of the test.
For the determination of the stability of the test item under the test conditions and of the maintenance of the test item concentration during the test period, duplicate samples from the test medium of the only test concentration and the control were collected at the end of the test (after 48 hours).

Test solutions

Vehicle:

yes

Details on test solutions:

s. attachement: "Test water"

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

Female Daphnia magna, clone 5; 2.75 to 22.25 hours old.
Source: The daphnids introduced in the test were taken from testing laboratory's in-house culture.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Post exposure observation period:

none

Test conditions

Test temperature:

20-21 °C

pH:

7.5-7.9

Dissolved oxygen:

8.7-9.0 mg/l

Nominal and measured concentrations:

100 mg/l (nominal)

Details on test conditions:

photoperiod: 16h light - 8h dark; light intensity: 870 - 1040 lux; and thus all parameters were within the ranges requested by guideline OECD 202

Results and discussion

Effect concentrations

Effect concentrations 1

Key result

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Basis for effect:

mobility

Effect concentrations 2

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Basis for effect:

mobility

Effect concentrations 3

Key result

Duration:

24 h

Dose descriptor:

NOEC

Effect conc.:

>= 100 mg/L

Nominal / measured:

nominal

Basis for effect:

mobility

Effect concentrations 4

Key result

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

>= 100 mg/L

Nominal / measured:

nominal

Basis for effect:

mobility

Effect concentrations 5

Key result

Duration:

24 h

Dose descriptor:

LOEC

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Basis for effect:

mobility

Effect concentrations 6

Key result

Duration:

48 h

Dose descriptor:

LOEC

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Basis for effect:

mobility

Details on results:

After 48 hours of exposure no immobilisation of the test animals was observed in the control or the only test concentration of nominal 100 mg test item/L.

Reported statistics and error estimates:

none

Any other information on results incl. tables

The quantification of the ammonium of the test item was performed using ion chromatography with conductivity detection (IC-CD).

At the start of the test 98%of the nominal test concentration was found. After 48 hours test duration, 93% of the nominal value was determined. During the test the Daphnia were exposed to a mean of 95 % of nominal. Therefore, all reported results refer to nominal concentrations.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The toxic effect of the test item Ammoniumhexafluorosilicate to Daphnia magna was assessed in a static limit-test. The 48-hour NOEC was determined to be >= 100 mg test item/L. The 48-hour LOEC was determined to be > 100 mg test item/L The 48-hour EC5o value was determined to be > 100 mg test item/L.

Executive summary:

The purpose of this study was to evaluate the influence of the test item Ammoniumhexafluorosilicate on the mobility ofDaphnia magna.Young daphnids (< 24 hours old) were exposed in a static test for 48 hours to test water containing the test item at the concentration of nominal 100 mg test item/L. This limit test was performed in compliance with the test guidelines to demonstrate that the test item has no toxic effect on daphnids up to at least the test concentration of 100 mg test item/L.