Registration Dossier
Registration Dossier
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EC number: 258-887-7 | CAS number: 53956-04-0
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 018
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-E (Determination of the "Ready" Biodegradability - Closed Bottle Test)
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Ammonium glycyrrhizate
- EC Number:
- 258-887-7
- EC Name:
- Ammonium glycyrrhizate
- Cas Number:
- 53956-04-0
- Molecular formula:
- C42H65NO16
- IUPAC Name:
- ammonium glycyrrhizate
1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- natural water: freshwater
- Details on inoculum:
- River water was sampled from “La Mourachonne”, situated downstream of a plant treating domestic wastewater (Pegomas, France).
The river water was aerated for about 7 days before use as inoculum at the test temperature to reduce endogenous respiration. - Duration of test (contact time):
- 28 d
Initial test substance concentration
- Initial conc.:
- 2 mg/L
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- Dissolved [02] (DO): Determinations of the concentration of dissolved oxygen using an oxygen electrode in duplicate vessels taken from each group (except the procedure and toxicity control) were made at day 0, 7, 14, 21 and 28 days, and only at the start of the test and after 7 and 14 days of incubation for the procedure and toxicity control.
Reference substance
- Reference substance:
- other: Sodium benzoate
Results and discussion
% Degradation
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 75
- Sampling time:
- 28 d
- Details on results:
- Test item AMMONIUM GLYCYRRHIZINATE was biodegraded by 75% (mean) at day 28 in the closed bottle test. The pass level of 60% was reached after less than 8 days of incubation upon achieving 10% biodegradation.
BOD5 / COD results
- Results with reference substance:
- The biodegradation percentage of the reference substance was 81% at day 14, confirming the suitability of the inoculum used.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
- Conclusions:
- The test item was biodegraded at 75% on day 28. The test item is thus readily biodegradable.
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