Registration Dossier
Registration Dossier
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EC number: 201-851-2 | CAS number: 88-68-6
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Non-guideline study. Limitations in design and/or reporting but outcome considered acceptable for assessment.
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- No details on method (other than basic test conditions) given.
- GLP compliance:
- no
- Analytical monitoring:
- yes
- Details on test solutions:
- stock solution: 5000 mg/L
- Test organisms (species):
- Pimephales promelas
- Details on test organisms:
- TEST ORGANISM
- Common name: fathead minnow
- Age: 31d
- Length: 18 mm
- Loading: 1.674 g/L
- Weight: 0.093 g - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Hardness:
- 46 mg/L CaCO3
- Test temperature:
- 25.8 °C
- pH:
- 7.54
- Dissolved oxygen:
- 7.2 mg/L
- Nominal and measured concentrations:
- - Nominal concentrations: 0 (control), 184, 283, 436, 670 and 1030 mg/L
- Measured concentrations (average): <5, 199, 275, 401, 631 and 920 mg/L. - Details on test conditions:
- TEST SYSTEM
- Test vessel: 1-L glass aquaria
- loading: 20 fish per L - Reference substance (positive control):
- no
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 395 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: CL: 354-439 mg/L
- Details on results:
- - Deaths observed at the nominal test concentrations of 184 and 283 mg/L were possibly due to handling at the onset of the test.
- Affected fish were hyperactive and lost equilibrium prior to death. - Sublethal observations / clinical signs:
Cumulative mortality
Nominal conc. (mg/L)
Number of fish
Mortality (cumulated) after
1 h
24 h
48 h
72 h
96 h
0
20
0
0
0
0
0
184
20
0
0
1
1
1
283
20
0
0
1
1
1
436
20
0
0
9
11
11
670
20
0
19
19
19
19
1030 20 0 20 20 20 20
Reference
Description of key information
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 395 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
