Registration Dossier
Registration Dossier
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EC number: 609-221-2 | CAS number: 3625-46-5
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 002
- Report date:
- 2002
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- GLP compliance:
- yes
Test material
- Reference substance name:
- 3,7,11-trimethyldodec-1-en-3-ol
- EC Number:
- 609-221-2
- Cas Number:
- 3625-46-5
- Molecular formula:
- C15H30O
- IUPAC Name:
- 3,7,11-trimethyldodec-1-en-3-ol
- Details on test material:
- - Name of test material (as cited in study report): Tetrahydronerolidol (THNER):
- Analytical purity: 96%
- Lot/batch No.: : Anlage 067, 13.06.01
water solubiluty 15 mg/L
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- Inoculum: Municipal activated sludge from laboratory wastewater treatment plants fed with municipal sewage . Concentration of dry substance 30 mg/l
- Duration of test (contact time):
- 42 d
Initial test substance concentration
- Initial conc.:
- 100 mg/L
- Based on:
- test mat.
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Composition of medium: according to OECD 301F
- Test temperature: 22 +/- 1°C
- pH: 7.6 -8.8
- pH adjusted: yes
- Aeration of dilution water:
- Suspended solids concentration: 30 mg/l dry substance
- Continuous darkness: yes
TEST SYSTEM
- Culturing apparatus: Sapromat
- Number of culture flasks/concentration: 8
- Method used to create aerobic conditions: aeration
SAMPLING
- Sampling frequency: daily
- Sample storage before analysis: no
CONTROL AND BLANK SYSTEM
- Inoculum blank: 1
- Abiotic sterile control: 1
- Toxicity control: 1
Reference substance
- Reference substance:
- aniline
Results and discussion
% Degradationopen allclose all
- Parameter:
- % degradation (O2 consumption)
- Value:
- 0 - 10
- Sampling time:
- 5 d
- Parameter:
- % degradation (O2 consumption)
- Value:
- 10 - 20
- Sampling time:
- 6 d
- Parameter:
- % degradation (O2 consumption)
- Value:
- 50 - 60
- Sampling time:
- 16 d
- Parameter:
- % degradation (O2 consumption)
- Value:
- 60 - 70
- Sampling time:
- 28 d
- Parameter:
- % degradation (O2 consumption)
- Value:
- 70 - 80
- Sampling time:
- 42 d
BOD5 / COD results
- Results with reference substance:
- Kinetic of control substance (in %):
day 1: 0 %
Day 4: 0 %
Day 5: 50-60 %
Day 14: 70-80 %
Day 42: 70-80 %
Applicant's summary and conclusion
- Interpretation of results:
- other: Biodegradable (not readily biodegradable according to OECD criteria)
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