Ethyl 4-[[(methylphenylamino)methylene]amino]benzoate

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

From December 14, 2016 to February 28, 2017

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

yes

Test organisms (species):

Oncorhynchus mykiss (previous name: Salmo gairdneri)

Details on test organisms:

- Source: Aqua Farms, Langley, BC
- Fork Length at study initiation: 3.8-4.7 cm
- Weight at study initiation: 0.34-0.85 g

Test type:

semi-static

Water media type:

freshwater

Total exposure duration:

96 h

Test temperature:

14-15°C

Dissolved oxygen:

77.4 -99.8% saturation

Nominal and measured concentrations:

Nominal concentrations: 0.375, 0.75, 1.5, 3 and 6 mg/L

Details on test conditions:

- Test vessel: 9 L glass jars
- Test volume: 8 L
- Aeration: ~6.5 mL/(min·L)
- Photoperiod: 16-hours light : 8-hours dark, with a 30 minute transition

Reference substance (positive control):

yes

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

1.4 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Sublethal observations / clinical signs:

Laboratory (negative) Control: dilution water

Solvent Control: 4.8 mL methanol in dilution water

 

Table 1. Measured Concentrations of Total Organic Carbon (TOC) in Test Solution

Nominal Test Item Concs. (mg/L)	Day 0 TOC (mg/L)		Day 1 TOC (mg/L)		Day 2 TOC (mg/L)		Day 3 TOC (mg/L)
	Initial	Final	Initial	Final	Initial	Final	Initial	Final
Laboratory Control	<0.50	0.83	1.19	<0.50	<0.50	0.76	<0.50	<0.50
Solvent Control	183	182	184	167	174	195	187	194
0.375	14.6	13.6	15.0	13.0	10.4	12.0	11.8	11.7
0.75	29.8	30.8	22.8	21.2	20.8	23.8	23.4	22.7
1.5	42.3	45.1	44.5	41.3	46.5	42.9	43.5	41.3
3	91.3	103	95.2	83.2	N/A	N/A	N/A	N/A
6	176	178	N/A	N/A	N/A	N/A	N/A	N/A

Table 2. Initial/Freshly Prepared Test Solution Mean Water Quality Measurements

Nominal Test Item Concentrations (mg/L)	Conductivity (μS/cm)	Mean Dissolved Oxygen (% Saturation)	Mean Temperature (°C)	Mean pH
Laboratory Control	28-32	99.0	14.1	7.3
Solvent Control	28-32	98.7	14.3	7.4
0.375	28-32	98.4	14.3	7.4
0.75	28-32	98.8	14.2	7.4
1.5	28-32	98.7	14.2	7.4
3	28-31	98.6	14.2	7.2
6	28	99.8	14.0	7.2

Table 3. Final/Old Test Solution Mean Water Quality Measurements

Nominal Test Item Concentrations (mg/L)	Mean Dissolved Oxygen (% Saturation)	Mean Temperature (°C)	Mean pH
Laboratory Control	86.9	14.8	7.2
Solvent Control	88.7	14.7	7.2
0.375	91.3	14.6	7.2
0.75	89.5	14.7	7.1
1.5	87.3	14.8	7.1
3	84.4	14.7	7.0
6	90.7	14.6	6.9

Table 4. Cumulative Mortalities of Rainbow Trout Over a 96 hr Exposure Period

Nominal Test Item Concentrations (mg/L)	24 hr (# / %)	48 hr (# / %)	72 hr (# / %)	96 hr (# / %)
Laboratory Control	0 / 0	0 / 0	0 / 0	0 / 0
Solvent Control	0 / 0	0 / 0	0 / 0	0 / 0
0.375	0 / 0	0 / 0	0 / 0	0 / 0
0.75	0 / 0	0 / 0	0 / 0	0 / 0
1.5	2 / 20	5 / 50	6 / 60	6 / 60
3	1 / 10	10 / 100	10 / 100	10 / 100
6	10 / 100	10 / 100	10 / 100	10 / 100

Table 5. Test Endpoint at 96 hour Based on Nominal Test Item Concentrations

Time Points	LC50 (95% CL) (mg/L)	Calculation Method
96 hr	1.4 (1.1, 1.7)	Untrimmed Spearman-Kärber

Validity criteria fulfilled:

yes

Conclusions:

According to OECD 203 test method, the 96-hr LC50 of Eversorb EP4 was 1.4 mg/L.

Executive summary:

This test using the procedures outlined in the Maxxam Study Plan for TOX1612 which is based on the SOP for the OECD 203 (BBY2 SOP-00018) and OECD 203 (OECD, 1992). The results of this OECD 203 test for Eversorb EP4 show that all validity criteria were met.

The values of water quality measurements were maintained constant condition for testing. The test endpoint was calculated based on the nominal test item concentrations and the results of the TOC analyses were reported. Under the conditions of this test, the 96-hr LC50 of Eversorb EP4 was 1.4 mg/L.

 

Description of key information

The 96-hr LC50 of Eversorb EP4 was 1.4 mg/L for Oncorhynchus mykiss (OECD TG203).

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

1.4 mg/L

Additional information

The values of water quality measurements contain dissolved oxygen concentrations (77.4%-99.8% of air saturated value), temperature (14.0-14.8) and pH (6.9-7.4)were maintained constant condition for testing. The mortality in the control did not exceed 10% at the end of the test. All test validity criteria were met. The test endpoint was calculated based on the nominal test item concentrations and the results of the TOC analyses were reported. Under the conditions of this test, the 96-hr LC50 of Eversorb EP4 was 1.4 mg/L.