Oct-1-ene-3-ol

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

The study was conducted between 30 July 2015 and 25 November 2015.

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2015

Report date:

2015

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)

Deviations:

no

Test guideline 2

Qualifier:

according to guideline

Guideline:

EPA OPPTS 835.3110 (Ready Biodegradability)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Oct-1-ene-3-ol

EC Number:

222-226-0

EC Name:

Oct-1-ene-3-ol

Cas Number:

3391-86-4

Molecular formula:

C8H16O

IUPAC Name:

oct-1-en-3-ol

Test material form:

liquid

Specific details on test material used for the study:

Lot No.: VE00345297
Aspect: colourless to pale yellow liquid
Purity: 99.7%

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

sewage, predominantly domestic, non-adapted

Details on inoculum:

Fresh activated sludge from a biological waste water treatment plant treating predominantly domestic sewage (Bois-de-Bay, Satigny, Switzerland) was used.
The sludge is collected in the morning, washed three times in the mineral medium (by centrifuging at 1000 g for 10 minutes, discarding the supernatant and resuspending in mineral medium) and kept aerobic until being used on the same day.

Duration of test (contact time):

62 d

Initial test substance concentration

Initial conc.:

30 mg/L

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

O2 consumption

Reference substance

Reference substance:

benzoic acid, sodium salt

Results and discussion

% Degradation

% Degradation 1

Parameter:

% degradation (O2 consumption)

Value:

34

Sampling time:

5 d

Remarks on result:

other: test concentration of 30 mg/L

% Degradation 2

Parameter:

% degradation (O2 consumption)

Value:

69

Sampling time:

15 d

Remarks on result:

other: test concentration of 30 mg/L

% Degradation 3

Key result

Parameter:

% degradation (O2 consumption)

Value:

80

Sampling time:

28 d

Remarks on result:

other: test concentration of 30 mg/L

Any other information on results incl. tables

RESULTS

Oxygen uptakes, as read on the Oxitop controller, are corrected to account for the small differences between actual and nominal concentrations of test and reference substances.

Calculated % biodegradation curves and detailed in the table below:

O₂uptake (mg O₂/L, adjusted to nominal concentrations):

	Days:		3	4	5	14	15	28	62
O2 uptake of sludge (inoculum blank)	3	B1	12.8	12.8	14.9	21.3	21.3	25.6	27.7
	4	B2	12.7	12.7	14.8	21.2	19.0	25.4	27.5
	mean	B	12.8	12.8	14.9	21.3	20.2	25.5	27.6
O2 uptake of Test Subst. + sludge	13	C1	8.2	24.5	47.0	81.9	81.9	94.1	100.1
	14	C2	2.1	12.3	41.1	76.0	78.0	94.4	104.9
O2 uptake of Test Substance		C1-B	-4.6	11.8	32.2	60.6	61.7	68.6	72.5
		C2-B	-10.6	-0.4	26.2	54.7	57.8	68.9	77.3
% biodegradation of test substance		D1	-5	14	37	70	72	80	84
		D2	-12	-1	30	64	67	80	90
	mean	D	-9	7	34	67	69	80	87

Calculations:

B1, B2, C1, C2, A1, A2, E1: experimental O₂uptake values

B = (B1 + B2)I2

D1 = 100 * (C1- B) /ThOD * [S]

D2 = 100 * (C2- B) /ThOD * [S]

D = (D1 + D2)/2

[S] : Initial test substance concentration (mg/L)

 

Degradation of sodium benzoate exceeded 40% after 7 days and 65% after 14 days: the activity of the inoculum was thus verified (validity criterion).

The repeatability validity criterion (not more than 20% difference between replicates) is fulfilled. Therefore, the test is considered valid.

 

COMMENTS AND CONCLUSIONS

The Ready Biodegradability of Amyl Vinyl Carbinol was determined by the Monometric Respirometry Test.

Amyl Vinyl Carbinol undergoes 80% biodegradation after 28 days (34% biodegradation on day 5 and 69% on day 15).

Amyl Vinyl Carbinol did inhibit the intrinsic respiration of the inoculum at the test concentration at the beginning of the test. Toxicity towards the inoculum at test concentration can therefore not be excluded.

Amyl Vinyl Carbinol should be regarded as readily biodegradable according to this test.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

readily biodegradable

Conclusions:

Amyl Vinyl Carbinol undergoes 80% biodegradation after 28 days in the test conditions. The 10-day window criterion is also fulfilled (34% biodegradation on day 5 and 69% on day 15).
Amyl Vinyl Carbinol did inhibit the intrinsic respiration of the inoculum at the test concentration at the beginning of the test. Toxicity towards the inoculum at test concentration can therefore not be excluded.
Amyl Vinyl Carbinol should be regarded as readily biodegradable according to this test.

Executive summary:

The Ready Biodegradability of Amyl Vinyl Carbinol was determined by the Manometric Respirometry Test according to OECD Guideline for the Testing of Chemicals No. 301F, Paris, 1992.

Amyl Vinyl Carbinol undergoes 80% biodegradation after 28 days. The 10-day window criterion is also fulfilled (34% biodegradation on day 5 and 69% on day 15).

Amyl Vinyl Carbinol did inhibit the intrinsic respiration of the inoculum at the test concentration at the beginning of the test. Toxicity towards the inoculum at test conentration can therefore not be excluded.

Amyl Vinyl Carbinol should be regarded as readily biodegradable according to this test.