N-benzyl-4-methoxy-α-methylphenethylamine

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

from 22 April to 26 July 1999

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1999

Report date:

1999

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Test guideline 3

Qualifier:

according to guideline

Guideline:

ISO 6341 (Water quality - Determination of the Inhibition of the Mobility of Daphnia magna Straus (Cladocera, Crustacea))

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

N-benzyl-4-methoxy-α-methylphenethylamine

EC Number:

256-155-1

EC Name:

N-benzyl-4-methoxy-α-methylphenethylamine

Cas Number:

43229-65-8

Molecular formula:

C17H21NO

IUPAC Name:

N-benzyl-1-(4-methoxyphenyl)propan-2-amine

Test material form:

other: liquid

Details on test material:

- Name of test material (as cited in study report): TH 1165 II
- Density: 1.05 g/ml (determined at NOTOX)
- Physical state: Yellow liquid
- Analytical purity: 98.2 % (area)
- Lot/batch No.: 61
- Expiration date of the lot/batch: 8 April 2000 (allocated by NOTOX, 1 year after receipt of the test substance)
- Stability under test conditions: Not indicated
- Storage condition of test material: At room temperature in the dark

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

The standard test procedures required generation of test solutions containing completely dissolved test substance concentrations or stable and homogeneous mixture or dispersions. The testing of concentrations that would disturb the test system was prevented (e. g. film of the test substance on the water surface).
Preparation started with a stock solution at 100 mg/l. Treatment with ultrasonic waves for 30 minutes was applied to dissolve the test substance in the test medium. The various test concentrations were prepared by subsequent dilutions of the the 100 mg/l stock in test medium. The final test solutions were all clear and colourless.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna (Crustacea, Cladocera)
- Age at study initiation (mean and range, SD): < 24 hours

- Method of breeding: The daphnia were bred with new-born animals, i. e. less than 3 days old, by placing about 250 of them into 10 litres of medium in an all-glass culture vessel. The maximum age of the cultures were 4 weeks. The renewal of the cultures were performed after 7 days of cultivation half of the medium twice a week. The temperature of the medium was hold at 18 - 22°C. Feeding was done daily, a suspension of fresh water algae. Breeding was performed in a M7medium, as prescribed by Dr. Elendt-Schneider (Elendt, B.-P., 1990)

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Test conditions

Hardness:

no data

Test temperature:

The temperature of the test medium measured in the blank control varied from 20.9 to 21.6ºC.

pH:

8.5

Dissolved oxygen:

8.93 mg O2/L

Salinity:

not applicable

Nominal and measured concentrations:

nominal concentration: 100 mg/l

Details on test conditions:

TEST SYSTEM
- Test vessel: 100 ml, all-glass
- Aeration: No aeration of the test solutions
- No. of organisms per vessel: 20 per concentration and 10 per vessel containing 80 ml medium
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2 blank control

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: ISO medium, prepared in milli-RO water

OTHER TEST CONDITIONS
- Photoperiod: 16 hours photoperiod daily

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Immobility (including mortality) at 24 hours and 48 hours

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 10 for the range finding test
- Range finding study: 0.1, 1.0, 10, 100 mg/l of the test substance
- Test concentrations: 2.2, 4.6, 10, 22, 46 mg/l of the test substance

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

4.9 mg/L

Nominal / measured:

nominal

Basis for effect:

mobility

Effect concentrations 2

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

6.1 mg/L

Nominal / measured:

nominal

Basis for effect:

mobility

Effect concentrations 3

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

2.2 mg/L

Nominal / measured:

nominal

Basis for effect:

mobility

Any other information on results incl. tables

Acute immobilisation of daphnia after 24 and 48 hours in the final EC50 -test:

 

Concentration TH 1165 II (mg/l)	Vessel number	Number Daphnia exposed	Response at 24 h		Response at 48 h
			number	%	number	%
Blank-control	A	10	0	0	0	0
	B	10	0	0	0	0
2.2	A	10	0	0	0	0
	B	10	0	0	0	0
4.6	A	10	2	20	3	30
	B	10	1	10	6	60
10	A	10	8	80	9	90
	B	10	10	100	10	100
22	A	10	10	100	10	100
	B	10	10	100	10	100
46	A	10	10	100	10	100
	B	10	10	100	10	100

 

 

 

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

Under the conditions of the present study, TH 1165 II did not induce acute immobilisation of Daphnia Magna at 2.2 mg/l after 48 hours of exposure (NOEC).
The 24h-EC50 was 6.1 mg/l with a 95% confidence interval between 5.2 and 7.5 mg/l.
The 48h-EC50 was 4.9 mg/l with a 95% confidence interval between 4.3 and 6.0 mg/l.