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EC number: 227-565-8 | CAS number: 5891-21-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 4th April 2017 to 7th July 2017
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Justification for type of information:
- Guideline study to GLP certification with no deviations.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 017
- Report date:
- 2017
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)
- Deviations:
- no
- Principles of method if other than guideline:
- Guideline study
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 5-chloropentan-2-one
- EC Number:
- 227-565-8
- EC Name:
- 5-chloropentan-2-one
- Cas Number:
- 5891-21-4
- Molecular formula:
- C5H9ClO
- IUPAC Name:
- 5-chloropentan-2-one
- Test material form:
- liquid
- Remarks:
- dark brown
Constituent 1
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source and lot/batch number of test material:
5-Chloropentan-2-one (CAS-No.: 5891-21-4), Sanofi Chimie
- Purity: 95.9%
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations:
Nominal concentrations were 1, 3.2, 10, 32, 100 mg/L
Geometric mean concentrations were 0.43, 1.38, 4.3, 13.3, 45.9 mg/L
- Sampling method:
Samples collected from all test concentrations for analysis at 0 and 72 hours.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
The test media were prepared just before introduction of the algae (= start of the test).
Test organisms
- Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- TEST ORGANISM
- Common name: Green algae
- Strain: Strain No. 61.81 SAG
- Age of inoculum (at test initiation): 4 days
Study design
- Test type:
- static
- Water media type:
- freshwater
- Remarks:
- OECD TG 201 medium
- Limit test:
- no
- Total exposure duration:
- 72 h
- Remarks on exposure duration:
- None
- Post exposure observation period:
- None
Test conditions
- Hardness:
- No data (OECD TG 201 medium)
- Test temperature:
- 23.0 – 23.6 °C
- pH:
- 8.0 - 8.1 at test start and 4.5 - 9.9 at test end
- Dissolved oxygen:
- No data (OECD TG 201 medium)
- Salinity:
- Not applicable
- Conductivity:
- No data (OECD TG 201 medium)
- Nominal and measured concentrations:
- Nominal concentrations were 1, 3.2, 10, 32, 100 mg/L
Geometric mean concentrations were 0.43, 1.38, 4.3, 13.3, 45.9 mg/L - Details on test conditions:
- TEST SYSTEM
- Initial cells density: approx. 5000 algal cells per mL test medium
- Control end cells density: 1.26 * 10^6 cells/ml
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 6
GROWTH MEDIUM
- Standard medium used: yes (OECD TG 201)
OTHER TEST CONDITIONS
- Photoperiod: Continuous illumination - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate (K2Cr2O7)
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 45.9 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 45.9 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Remarks:
- yield
- Duration:
- 72 h
- Dose descriptor:
- EC20
- Effect conc.:
- > 45.9 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC20
- Effect conc.:
- 23.6 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Remarks:
- yield
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 41.8 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 16.1 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Remarks:
- yield
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 13.3 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 13.3 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Remarks:
- yield
- Key result
- Duration:
- 72 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 45.9 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 45.9 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Remarks:
- yield
- Details on results:
- - Exponential growth in the control (for algal test): yes
- Results with reference substance (positive control):
- - Results with reference substance valid? Yes
- Potassium dichromate 72 hour EC50 (growth inhibition): 1.12 mg/l - Reported statistics and error estimates:
- Based on the calculated cell densities, the 72 hour ErC50 (growth) and the 72 hours EyC50 (yield), the corresponding EC20 and EC10 values and where possible their 95 %-confidence limits were calculated by non-linear regression. For the determination of the 72 hours LOEC and the 72 hours NOEC, the calculated growth rates and yields at each test concentration were tested for significant differences compared to the control values by Williams t-test (yield and growth rate). The software used to perform the statistical analysis was ToxRat Professional, Version 3.2.1, ToxRat Solutions GmbH.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The reliable (Klimisch 1) OECD 201 Guideline study on algal growth inhibition reported a 72-hr EC50 of > 45.9 mg/l and an EC10 of 41.8 mg/L based on growth of Pseudokirchneriella subcapitata (measured geometric mean).
- Executive summary:
The reliable OECD 201 Guideline algal growth inhibition study was undertaken to GLP standards. The measured concentrations in the nominal test concentrations of 10, 3.2 and 1.0 mg test item/L were below the limit of quantification. All reported results refer to geometric mean concentrations, since the test item concentrations were not within ± 20% of the nominal concentrations during the test.
The following results for the test item were determined:
Endpoint NOEC LOEC EC10 EC20 EC50 Growth rate 13.3 mg/l 45.9 mg/l 41.8 mg/l >45.9 mg/l >45.9 mg/l Yield 13.3 mg/l 45.9 mg/l 16.1 mg/l 23.6 mg/l >45.9 mg/l
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