Phosphonium, [5-(4-hydroxy-2,6,6-trimethyl-3-oxo-1-cyclohexen-1-yl)-3-methyl-2,4-pentadien-1-yl]triphenyl-, bromide (1:1)

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1998

Report date:

1998

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

Version / remarks:

Cited as Directive 84/449/EEC, C.1 ("Acute toxicity for fish")

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

[(5-(4-hydroxy-2,6,6-trimethyl-3-oxo-1-cyclohexen-1-yl)-3-methyl-2,4-pentadienyl](triphenyl)phosphonium bromide

EC Number:

617-589-0

Cas Number:

84592-32-5

Molecular formula:

C33 H36 O2 P .Br

IUPAC Name:

[(5-(4-hydroxy-2,6,6-trimethyl-3-oxo-1-cyclohexen-1-yl)-3-methyl-2,4-pentadienyl](triphenyl)phosphonium bromide

Details on test material:

- Name of test material (as cited in study report): Asta-C15-Salz krist.
- Lot/batch No.: 95149-4B/Trommel 2
- Analytical purity: 98 g/100 g

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test concentrations were prepared separately by adding the required amount of test substance to test medium. The mixture was stirred using an ultra-turrax stirrer.

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: Zebra fish
- Source: Charles River Aquatics, Someren, Netherlands
- Age at study initiation (mean and range, SD): ca. 3 month
- Length at study initiation (length definition, mean, range and SD): 3.3 cm (range: 2.9 - 3.6 cm)
- Weight at study initiation (mean and range, SD): 0.35 g (0.2 - 0.5 g)


ACCLIMATION
- Acclimation period: 3 days
- Type and amount of food: TETRA MIN, Tetra Werke, Melle, Germany; ad libitum
- Feeding frequency: daily and on workdays generally additionally live artemia
- Health during acclimation (any mortality observed): no mortality observed

Study design

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Test temperature:

23 - 24 °C

pH:

8.0 - 8.5

Dissolved oxygen:

5.7 - 8.1 mg/l

Nominal and measured concentrations:

Nominal test substance concentrations:
0, 4.64, 10.0, 21.5, 46.4 and 100 mg/l

Analytically determined test concentrations after 24 hours:
<2, 1.9, 4.9, 10.9, 26.8, 58.2 mg/l
Analytically determined test concentrations after 96 hours:
3.0 (4.64 nominal), 6.4 (10.0 nominal), 14.2 (21.5 nominal) mg/l

Details on test conditions:

TEST SYSTEM
- Test vessel: glass aquaria (30 x 22 x 24 cm)
- Fill volume: 10 l
- Renewal rate of test solution (frequency/flow rate): renewal of test solution after 48 hours
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: 0.4 g/l

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: tap water of the city of Frankenthal/Germany


OTHER TEST CONDITIONS
- Photoperiod: 16:8 h day-night regime

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Mortality and symptoms of intoxication after 1, 4, 24, 48,72 and 96 hours

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2.2
- Results used to determine the conditions for the definitive study: LC50 (96 h): 10 - 100 mg/l

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

4.64 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 2

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

14.67 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: geom. mean of 21.5 -46.4 mg/l

Effect concentrations 3

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

46.4 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

At a test substance concentration of 21.5 mg/l apathy and swimnming near the bottom was obersved after 1 hour of exposure. At a concentration of 46.4 mg/l accelerated respiration, apathy, O :swimming near water surface and swimnming near the bottom were observed after 1 hours. At a test concentration of 100 mg/l a apathy and swimming near water surface was observed after 1 hour.

Reported statistics and error estimates:

Finney, D.J., Probit Analysis, Cambr. Univ. Press, 3rd ed., 1971