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Diss Factsheets
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EC number: 228-036-4 | CAS number: 6092-54-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Skin irritation / corrosion
Administrative data
- Endpoint:
- skin irritation / corrosion
- Remarks:
- in vitro
- Type of information:
- experimental study
- Adequacy of study:
- other information
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Guideline study, available as unpublished report, no restrictions, fully adequate for assessment
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 013
- Report date:
- 2013
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 431 (In Vitro Skin Corrosion: Human Skin Model Test)
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 439 (In Vitro Skin Irritation: Reconstructed Human Epidermis Test Method)
- Qualifier:
- according to guideline
- Guideline:
- other: Commission Regulation (EC) No 440/2008
- Qualifier:
- according to guideline
- Guideline:
- other: Commission Regulation (EU) Nº 640/2012
- GLP compliance:
- no
Test material
- Reference substance name:
- Hexyl chloroformate
- EC Number:
- 228-036-4
- EC Name:
- Hexyl chloroformate
- Cas Number:
- 6092-54-2
- Molecular formula:
- C7H13ClO2
- IUPAC Name:
- hexyl carbonochloridate
- Details on test material:
- - Name of test material (as cited in study report): n-Hexylchloroformate
- Physical state: liquid
Constituent 1
Test animals
- Species:
- other: EpiDerm™ tissues (reconstructed three dimensional human epidermis model)
Test system
- Vehicle:
- unchanged (no vehicle)
- Controls:
- other: negative and positive controls
- Amount / concentration applied:
- TEST MATERIAL
- Amount applied: 50 μL (corrosion test) or 30 μL (irritation test) - Duration of treatment / exposure:
- 3 minutes and 1 hour (corrosion test) or 1 hour with 42 hours post incubation (irritation test).
- Details on study design:
- The test consists of a single topical exposure of the test substance to the surface of a human reconstituted epidermis model for 3 minutes and 1 hour (corrosion test) or 1 hour with 42 hours post incubation (irritation test) followed by a cell viability test. Cell viability is measured by dehydrogenase conversion of the yellow, water-soluble MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide), present in cell mitochondria, into a blue formazan salt that is measured quantitatively after isopropanol –extraction from the tissues. The optical density of the extracts of test substance treated tissues is compared to negative control values from tissue treated with de-ionized water or PBS and is expressed as relative tissue viability.
- Negative control (NC): de-ionized water (corrosion test) / PBS, sterile (irritation test)
- Positive control (PC): 8 N KOH (corrosion test) / 5% SDS in de-ionized water, sterile (irritation test)
- The corrosion test was performed in duplicate.
- The irritation test in triplicate.
Evaluation criteria:
- Corrosion test (viability as % of negative control): 3 min: <50 = Corrosive; 3 min ≥50 and 1 hour <15 = Corrosive; 3 min: ≥ 50 and 1 hour ≥15 = Non-corrosive
- Irritation test (viability as % of negative control): ≤ 50 = Irritant; >50 = Non-irritant
Results and discussion
In vivo
Resultsopen allclose all
- Irritation parameter:
- other: Cell viablity
- Basis:
- mean
- Time point:
- other: 3 minutes exposure
- Score:
- 99
- Remarks on result:
- other: Corrosion test (% viablity relative to control)
- Irritation parameter:
- other: Cell viability
- Basis:
- mean
- Time point:
- other: 1 hour exposure
- Score:
- 97
- Remarks on result:
- other: Corrosion test (% viablity relative to control)
- Irritation parameter:
- other: Cell viabilty
- Basis:
- mean
- Time point:
- other: 1 h exposure, 42 hours postincubation
- Score:
- 104
- Remarks on result:
- other: Irritation test (% viablity relative to control)
Applicant's summary and conclusion
- Interpretation of results:
- not irritating
- Remarks:
- Migrated information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.