Zinc bis(dibenzyldithiocarbamate)

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Remarks:

Minor restrictions in design and/or reporting but otherwise adequate for assessment.

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

GLP compliance:

no

Analytical monitoring:

no

Vehicle:

yes

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances):

- Chemical name of vehicle (organic solvent, emulsifier or dispersant): DMSO

Test organisms (species):

Poecilia reticulata

Details on test organisms:

TEST ORGANISM
- Common name: guppy
- Source: Akzo Research Laboratories Arnhem, dpt. CRL.
- Age at study initiation: Young fish
- Length at study initiation: ≤ 2 cm
- The fish were kept at 23 ± 2 ºC

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Post exposure observation period:

None

Hardness:

13°dH

Test temperature:

23 ± 2°C

pH:

approximately 8.2

Salinity:

Not applicable

Nominal and measured concentrations:

Not known

Details on test conditions:

TEST SYSTEM
- Test vessel: 2L glass aquaria
- Material, size, headspace, fill volume: 2 l glass aquaria filled with 1 l of test medium.
- Aeration: The medium was aerated before use.
- No. of organisms per vessel: 7-10 fish per concentration
- Renewal rate of test solution : The test medium was refreshed after 48 hours.
- Biomass loading rate: The loading in the glass test vessels was approximately 0.6 g biomass/l.

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Synthetic medium, Dutch Standard Water according to NPR 6507
- Containing: 100 mg NaHCO3/l, 20 mg KHCO3/l, 200 mg CaCl2.2H2O/l and 180 mg MgSO4.7H2O/l
- Preparation of the test medium: A stock solution in DMSO was made, from which a second stock solution in dilution water was prepared.

Reference substance (positive control):

no

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 10 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

- Mortality of control: ≤ 10%

Description of key information

The 96-h LC50 of > 10 mg/L in Poecilia reticulata.

Additional information

One short-term toxicity study with freshwater fish is available for zinc bis(dibenzyldithiocarbamate). The test is a semi-static non-GLP, OECD 203 guideline study with Poecilia reticulata without analytical monitoring (Akzo Nobel Research Laboratories, 1991). The 96-h LC50 value is >10 mg/L.