Cerium trinitrate

Acute Toxicity: other routes

Administrative data

Endpoint:

acute toxicity: other routes

Type of information:

experimental study

Adequacy of study:

supporting study

Study period:

data not available

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study without detailed documentation

Cross-reference

Reason / purpose for cross-reference:

reference to same study

Data source

Reference

Reference Type:

publication

Title:

The Acute Mammalian Toxicity of Rare Earth Nitrates and Oxides

Author:

Bruce DW

Year:

1963

Bibliographic source:

Toxicol Appl Pharmac, 5, 750-759

Materials and methods

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

Cerium nitrate, hexahydrate

IUPAC Name:

Cerium nitrate, hexahydrate

Test material form:

not specified

Details on test material:

- Test material identity: Cerium nitrate, as cerium (III) nitrate hexahydrate
- Analytical purity: > 98%
- Impurities: No data
- Lot/batch No: No data
- Expiration date of the lot/batch: No data
- Stability under test conditions: No data
- Storage condition of test material: No data
- Other information: Ce(NO3)3.6H2O were obtained from the Lindsay Chemical Company, West Chicago, Illinois, and the K and K Laboratories, Jamaica, New York.

Test animals

Species:

rat

Strain:

Sprague-Dawley

Sex:

female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: no data available
- Age at study initiation: no data available
- Weight at study initiation: 190 g to 250 g
- Fasting period before study: no data available
- Housing: the animals were housed in air-conditioned quarters
- Diet (e.g. ad libitum): yes, ad libitum (food: Rockland rat diet or Rockland mouse pellets)
- Water (e.g. ad libitum): yes, ad libitum
- Acclimation period: no data available

ENVIRONMENTAL CONDITIONS
- Temperature (°C): no data available
- Humidity (%): no data available
- Air changes (per hr): no data available
- Photoperiod (hrs dark / hrs light):no data available

Administration / exposure

Route of administration:

intraperitoneal

Vehicle:

water

Details on exposure:

The solution was given in single dose to female rat (50-10 female/groups) at various doses by the intraperitoneal route in volumes equivalent to 0.1% of the total body weight.
Aqueous solutions (pH 4.0-5.9) were prepared immediately before injection.

No. of animals per sex per dose:

5 to 10 animals / groups

Details on study design:

- Duration of observation period following administration: 30 days

Results and discussion

Effect levels

Sex:

female

Dose descriptor:

LD50

Effect level:

290 mg/kg bw

95% CL:

238 - 358

Remarks on result:

other: Preliminary experiments indicated no sex difference in the susceptibility of rats to these metals given intraperitoneally.

Mortality:

Although no definite time-mortality pattern was observed, very few of the rats that received lethal doses died during the first 8 days, in contrast to mice.
The majority of animals that failed to survive the 30-day observation period succumbed during the period from 10 to 25 days after
injection.

Gross pathology:

Almost all the animals that died during this period had grossly distended abdomens, and edema of the limbs was observed in many of the animals that received the higher doses. Gross pathologic examination of the animals revealed an inflammatory condition in the peritoneal cavity with massive adhesions and accumulation of hemorrhagic ascitic fluid.

Applicant's summary and conclusion

Conclusions:

An LD50 value of 290 mg/kg was determined in females after intraperitoneal administration of the test substance.