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EC number: 446-220-4 | CAS number: 365411-50-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
Description of key information
A study was performed to assess the effect of the test material on the growth of the green alga Scenedesmus subspicatus according to OECD TG 201. The ECr50 was > 14.4 mg/l. An ECr10 or 1.9 mg/l was derived and a NOEC of 0.78 mg/l, based on measured concentrations. For the risk assessment the EC10 will be used.
Key value for chemical safety assessment
- EC10 or NOEC for freshwater algae:
- 1.9 mg/L
Additional information
A study was performed to assess the effect of the test material on the growth of the green alga Scenedesmus subspicatus.The method followed that described in the OECD Guidelines for Testing of Chemicals (1984) No 201, "Alga, Growth Inhibition Test" referenced as Method C.3 of Commission Directive 92/69/EEC (which constitutes Annex V of Council Directive 67/548/EEC).
Following a preliminary range-finding test, Scenedesmus subspicatus was exposed to solutions of the test material at nominal concentrations of 1.25, 2.5, 5.0, 10 and 20 mg/L (three replicate flasks per concentration) for 72 hours, under constant illumination and shaking at a temperature of 24 ± 1 °C. The test material solutions were prepared by stirring an excess (100 mg/L) of test material in culture medium using a propeller stirrer at approximately 2000 rpm at a temperature of 25 °C for 24 hours. After the stirring period any undissolved test material present was removed by filtration (0.2 µm Gelman Suporcap filter, first approximate 1 litre discarded in order to pre-condition the filter) to produce a saturated solution with a nominal concentration of 20 mg/L. This saturated solution was then further diluted, as necessary, to provide the remaining test groups.
Samples of the algal populations were removed daily and cell concentrations determined for each control and treatment group, using a Coulter® Multisizer Particle Counter. Results. Exposure of Scenedesmus subspicatus to the test material gave an ErC50(0 - 72 h) value of > 14.4 mg/l. The EC10 for growth rate was 1.9 mg/L. Both values are based on geometric mean concentrations.
Analysis of the test preparations at 0 hours showed the measured concentrations to be near nominal. After 72-Hours there was a marked decline in measured concentrations to 37 % to 53 % of nominal values. Stability analyses indicated that the test material was stable in aqueous medium over the test period and hence the decline in measured concentrations was considered to be due to adsorption to algal cells. Adsorption was not a factor in the stability analyses conducted as no algal cells were present. Given this decline in measured test concentrations it was considered justifiable to base the results on the geometric mean measured test concentrations in order to give a "worst case" analysis of the data.
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