4-anilino-3-nitro-N-phenylbenzenesulphonamide

Biodegradation in water: screening tests

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

supporting study

Study period:

1993

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Qualifier:

according to guideline

Guideline:

other: EG C.8 (Uber MITI-Test)

Deviations:

not specified

Principles of method if other than guideline:

In-house SOP A15

GLP compliance:

no

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge (adaptation not specified)

Details on inoculum:

No data

Duration of test (contact time):

5 d

Details on study design:

No data

Preliminary study:

No data

Test performance:

No data

Parameter:

BOD5

Value:

10 mg O2/g test mat.

Validity criteria fulfilled:

not specified

Interpretation of results:

not readily biodegradable

Conclusions:

BOD5 of test substance was found to be 10 mg O2/g.

Executive summary:

BOD5 of FAT 36014/F was determined according to in-house Ciba Geigy Methode A.15, which is comparable to internationally accepted guidelines. Based on these investigations, the BOD5 of test substance was found to be 10 mg O2/g. Considering the COD value determined with the test substance which is 1412 mg O2/g, the resulting BOD5/COD quotient is 0.007, indicating that the test substance is unlikely to be biodegradable.

Reference 2

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1993

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I))

Deviations:

yes

Remarks:

(instead of 28 d the test has been finished after 14 d since no degradation activity could be observed)

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

Code number: FAT 36014/F
EN/Batch: 292347.26
Purity: 90.7 %
Appearance: solid
Solubility: <1 g/L (in water)
Storage: room temperature
Expiration date: 04/98

Oxygen conditions:

aerobic

Inoculum or test system:

sewage, domestic (adaptation not specified)

Details on inoculum:

Strain / Inoculation: Mixture of polyvalent bacteria collected in the aeration tank of a domestic sewage treatment plant, ARA Basel Communal
Bacteria concentration: 30 mg/L

Duration of test (contact time):

14 d

Initial conc.:

100 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation:

O2 consumption

Reference substance:

benzoic acid, sodium salt

Preliminary study:

No data

Test performance:

No data

Parameter:

% degradation (O2 consumption)

Value:

0

Sampling time:

14 d

Details on results:

The biodegradation of test substance (corrected by the innoculum control) expressed as the % of BOD versus ThOD was 0 % (14 d).

Results with reference substance:

The biodegradation (corrected by the inoculum control) expressed as the % of BOD versus ThOD was 91 % (14 d).

Validity criteria fulfilled:

yes

Interpretation of results:

under test conditions no biodegradation observed

Conclusions:

Under the study conditions, the test substance was not readily biodegradable.

Executive summary:

The ready biodegradability of the test substance (of ca. 90.75 % purity) was determined in a 14 d MITI test (I) according to OECD Guideline 301C and the EU Method C.7.

Both the test substance and reference substance sodium benzoate were tested at a concentration of 100 mg/L. Biodegradation was equivalent to 0 and 91 %, respectively.

Under the study conditions, the test substance was not readily biodegradable.

Reference 3

Endpoint:

biodegradation in water: screening test, other

Remarks:

COD

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1993

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

EU Method C.6 (Degradation: Chemical Oxygen Demand)

Version / remarks:

DEV DIN 38'409 - H 41-1 (1980) corresponding to 92 / 96 / EEC C.6.

Deviations:

yes

Remarks:

Mean of 2 determinations instead of 3

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

Code number: 36014/F
EN/Batch: 292347.26
Purity: 90.7 %
Appearance: solid
Solubility: <1 g/L (in water)
Storage: Room temperature
Expiration date: 04/98

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge (adaptation not specified)

Details on inoculum:

None

Duration of test (contact time):

2 h

Parameter followed for biodegradation estimation:

O2 consumption

Details on study design:

Experimental conditions:
Sample weight: 7.39 ± 7.11 mg of the test substance
Temperature: 148 ± 3 °C
Duration: 2 hours
Estimation Technique: Titration (Potentiometric) METROHM Titroprozessor 670 (SAV : 00 111)

Reference substance:

other: Potassium hydrogen phthalate solution

Preliminary study:

None

Test performance:

None

Remarks on result:

not measured/tested

Details on results:

None

Parameter:

COD

Value:

1 412 mg O2/g test mat.

Results with reference substance:

The COD of Potassium hydrogen phthalate is (Criteria: 200 ± 8 mg O2/I): 201 mg O2/I

Validity criteria fulfilled:

not specified

Interpretation of results:

not readily biodegradable

Conclusions:

The COD of FAT-36014/F was found to be 1412 mg O2/g. The COD of the reference substance (potassium hydrogen pthalate) was found to be 201 mg O2/I

Executive summary:

A test was carried out according to DEV DIN 38'409 - H 41-1 (1980) corresponding to 92/96/EEC C.6. The test was performed in compliance with the Good Laboratory Practice (GLP) Regulations of Switzerland.

A predeterminated amount of the test substance FAT - 36014/F in water is oxidized by potassium dichromate in a strong sulphuric acid medium with silver sulphate as a catalyst under reflux for 2 hours. The residual dichromate is determined by titration with standardized ferrous ammonium sulphate.

The test substance was tested with a sample weight of 7.39 ± 7.11 mg. The reference substance Potassium hydrogen phthalate solution was tested in a concentration of 20 ml/I.

Based on the findings of the test, the COD of FAT-36014/F was calculated to be 1412 mg O2/g. The COD of the reference substance (potassium hydrogen pthalate) was found to be 201 mg O2/I.

Description of key information

The test item FAT 36014 was investigated for its ready biodegradability following OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I)). In this study both the test substance and reference substance sodium benzoate were tested at a concentration of 100 mg/L. Biodegradation was equivalent to 0 % and 91 %, respectively. Another test to determine the COD value was performed following EU Method C.6. The test substance was tested with a sample weight of 7.39 ± 7.11 mg. The reference substance potassium hydrogen phthalate solution was tested in a concentration of 20 ml/l. The COD of FAT-36014/F was found to be 1412 mg O2/g in comparison with the COD of the reference substance 201 mg O2/l. At the end of 44 days a study conducted following OECD Guideline 302 B (Inherent biodegradability: Zahn-Wellens/EMPA Test) found 59 % elimination. Few more studies rated as weight of evidence with Klimisch 4 reported COD: 1412 mg/O2 g, percent degradation in 14 days: 0 %. In the light of percentage degradation and COD data from all the above mentioned studies the test item FAT 36014 is considered as not biodegradable.

Key value for chemical safety assessment

Biodegradation in water:

under test conditions no biodegradation observed

Type of water:

freshwater