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EC number: - | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 28 January 2011 - 14 April 2011
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: According to OECD and in accordance with GLP
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 011
- Report date:
- 2011
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.11 (Biodegradation: Activated Sludge Respiration Inhibition Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- ISO 8192 (Water quality - Test for inhibition of oxygen consumption by activated sludge for carbonaceous and ammonium oxidation)
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Details on test material:
- Description Orange powder
Batch MB-1
Test substance storage At room temperature protected from light
Stability under storage conditions Stable
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- no
- Details on test solutions:
- The batch of E-Y110 tested was an orange powder with a purity of 95.5% and indicated to be light sensitive. Preparation was as much as possible performed under low light conditions.
A stock solution of 0.5 g/l was prepared by adding 125.34 mg test substance to 250 ml of Milli-RO water (tap water purified by reverse osmosis; Millipore Corp., Bedford, Mass., USA). Thorough mixing (vortex) was used to accelerate dissolution and to ensure homogeneity. Volumes of the orange stock solution corresponding to the test concentration were then added to the test media.
A concentration of 100 mg/l was tested in duplicate.
Test organisms
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- The sludge was coarsely sieved, washed and diluted with ISO-medium. A small amount of the sludge was weighed and dried overnight at ca. 105°C to determine the amount of suspended solids (4.0 g/l of sludge, as used for the test). The pH was 7.6 on the day of testing. The batch of sludge was used one day after collection; therefore 50 ml of synthetic sewage feed was added per litre of activated sludge at the end of the collection day. The sludge was kept aerated at test temperature until use.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 3 h
- Post exposure observation period:
- Oxygen consumption was measured and recorded for approximately 10 minutes.
Test conditions
- Test temperature:
- The temperature of the test medium was between 19.4 and 19.9°C
- pH:
- Controls: 7.6-7.8
Test substance series: 7.7-7.8 - Dissolved oxygen:
- Oxygen consumption:
Controls series:54-56 mg O2/l/h
Test substance series: 49-51 mg O2/l/h - Nominal and measured concentrations:
- A nominal concentration of 100 mg/L was tested in duplicate
- Details on test conditions:
- TEST SYSTEM
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: All glass, approximately 300 ml oxygen bottles (measuring) and
1 litre test bottles(exposure)
- Aeration:During exposure with clean, oil-free air
- No. of vessels per concentration (replicates):2
- No. of vessels per control (replicates):2/ series
- Biomass loading rate:4 g/l of sludge as used for the test
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Tap-water purified by reverse osmosis (Milli-RO
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : The respiration rate from each vessel, in mg O2/l/hr, was
calculated from the linear part of the respiration curve, which was generally between 2.5 and 6.5 mg O2/l.
TEST CONCENTRATIONS
- Test concentrations: limit study at a loading rate of 100 mg/l - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 3 h
- Dose descriptor:
- IC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 3 h
- Dose descriptor:
- IC10
- Effect conc.:
- ca. 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Remarks on result:
- other: 7 and 11% inhibition at 100 mg/l
- Details on results:
- 7 and 11% inhibition of respiration rate of the sludge was recorded at 100 mg E-Y110 per litre in the duplicate measurements. Therefore, no further testing was needed. Hence, the EC50 of E-Y110 exceeded 100 mg/l (based on nominal concentrations).
- Results with reference substance (positive control):
- Results with reference substance valid: yes
The inhibitory effect of 3,5-dichlorophenol on aerobic waste water (activated sludge) bacteria increased with increased with increasing
concentration , ranging from a 38 % inhibition at 5.0 mg/l to 83% at 30 mg/l. Relevant effects levels: The EC50 of 3,5-dichlorophenol was: 7.0 mg/l. - Reported statistics and error estimates:
- For the reference substance the percentage inhibition was plotted against the logarithm of the concentrations and the EC50 was determined using
linear regression analysis.
For E-Y110 no EC50 could be calculated because the test substance proved to be non-toxic (EC50 >100 mg/l).
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- Under the conditions of this present test, E-Y110 was slightly inhibitory to waste water (activated sludge) bacteria at a nominal concentration of 100 mg/l (7 and 11% inhibition of respiration rate of the sludge). The 3-hour EC50 of E-Y110 exceeded a nominal concentration of 100 mg/l.
- Executive summary:
The influence of E-Y110 on the respiration rate of activated sludge was investigated after a contact time of 3 hours. The study procedures described in this report were based onthe OECD guideline No. 209, 1984.In addition, the procedures were designed to meet the test methods of the CouncilRegulation (EC) No. 440/2008 of, Publication No. L142, Part C11 and ISO Standard 8192 (2007). A concentration of 100 mg/l was tested in duplicate. Two controls without test substance were tested in each test series. Optimal contact between the test substance and test medium was ensured applying continuous aeration and stirring during the 3-hour exposure period. Thereafter, oxygen consumption was measured and recorded for approximately 10 minutes. 7 and 11% inhibition of respiration rate of the sludge was recorded at 100 mg E-Y110 per litre in the duplicate measurements. Therefore, no further testing was needed. Hence, the EC50of E-Y110 exceeded 100 mg/l (based on nominal concentrations). The batch of activated sludge was checked for sensitivity by testing the reference substance 3,5-dichlorophenol. Since all criteria for acceptability of the test were met, this study was considered to be valid.
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