o-fluoro-α,α,α-trichlorotoluene

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Remarks:

Type of genotoxicity: gene mutation

Type of information:

experimental study

Adequacy of study:

key study

Study period:

August 20 -23, 1996

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1996

Report date:

1996

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 471 (Bacterial Reverse Mutation Assay)

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method B.13/14 (Mutagenicity - Reverse Mutation Test Using Bacteria)

GLP compliance:

yes

Type of assay:

bacterial reverse mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

o-fluoro-α,α,α-trichlorotoluene

EC Number:

207-692-5

EC Name:

o-fluoro-α,α,α-trichlorotoluene

Cas Number:

488-98-2

Molecular formula:

C7H4Cl3F

IUPAC Name:

1-fluoro-2-(trichloromethyl)benzene

Method

Target gene:

hystidine locus

Species / strain

Species / strain / cell type:

S. typhimurium, other: TA98, TA100

Metabolic activation:

with and without

Metabolic activation system:

S9

Test concentrations with justification for top dose:

The maximum concentration was 5000 ug/plate. The concentration range covered two logarithmic decades.
According to the dose selection criteria the test article was tested at the following concentrations:
3.3; 10; 33.3; 100; 333.3; 1000; 2500 and 5000 ug/plate.

Controls

Untreated negative controls:

yes

Negative solvent / vehicle controls:

yes

Positive controls:

yes

Positive control substance:

sodium azide

other: 2-aminoanthracene and 4-nitro-o-phenylene-diamine

Results and discussion

Test results

Test results 1

Species / strain:

S. typhimurium TA 98

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

cytotoxicity

Remarks:

from 1000 to 5000 ug/plate

Test results 2

Species / strain:

S. typhimurium TA 100

Metabolic activation:

with and without

Genotoxicity:

positive

Cytotoxicity / choice of top concentrations:

cytotoxicity

Remarks:

from 1000 to 5000 ug/plate

Additional information on results:

Toxicity evident as a reduction in the number or revertants, was observed in TA98 (+/- S9 mix) and in TA100 (- S9 mix) from 1000 ug/plate up to 5000 ug/plate.
The plates incubated with the test article showed reduced background growth at 333.3 ug/plate and above in strain TA98 and at 1000 ug/plate and above in the absence and at the maximal concentration in the presence of S9 mix in strain TA100.
An increase in revertant colony numbers with factors exceeding the threshold of 2.0 was observed following treatment with test item in strain TA100 at 100 and 333.3 ug/plate without S9 mix and from 1000 up to 5000 ug/plate with S9 mix. At concentrations above 333.3 toxic effects result in a decrease of the number of revertant colonies with a concomitant decrease of the corresponding factor.

Remarks on result:

other: all strains/cell types tested

Remarks:

Migrated from field 'Test system'.

Applicant's summary and conclusion

Conclusions:

Interpretation of results (migrated information):
positive

In conclusion, it can be stated that during the described mutagenicity test and under the experimental conditions reported, the test article induced gene mutations by base pair changes genome of the strain TA 100.
Therefore, the test item is considered to be mutagenic in this Salmonella tymphimurium reverse mutation assay.