2,4-dichloro-1-(trichloromethyl)benzene

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1986-10-14 to 1986-12-09

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1986

Report date:

1986

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 401 (Acute Oral Toxicity)

GLP compliance:

yes

Test type:

standard acute method

Test material

Test material information

Constituent 1

Reference substance name:

2,4-Dichlorobenzotrichloride

IUPAC Name:

2,4-Dichlorobenzotrichloride

Constituent 2

Reference substance name:

2,4-dichloro-1-(trichloromethyl)benzene

EC Number:

235-868-1

EC Name:

2,4-dichloro-1-(trichloromethyl)benzene

Cas Number:

13014-18-1

Molecular formula:

C7H3Cl5

IUPAC Name:

2,4-dichloro-1-(trichloromethyl)benzene

Test material form:

solid: crystalline

Details on test material:

Please refer to confidential details on test material

Test animals

Species:

rat

Strain:

Wistar

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: HOECHST AG, Kastengrund, SPF-Zucht
- Age at study initiation: male ca. 7 wks, female ca. 8 wks
- Weight at study initiation: males 169 g - 202 g (average 188 g); females: 178 g - 195 g (average 195 g)
- Fasting period before study: about 16 hours
- Housing: 5 animals per makrolon cage
- Diet: Altromin 1324 rat diet, ad libitum
- Water (e.g. ad libitum): tap water, ad libitum
- Acclimation period: at least 5 days


ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22 +/- 3
- Humidity (%): 50 +/- 20
- Air changes (per hr): no data
- Photoperiod: 12 hrs dark / 12 hrs light

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

other: sesame oil

Details on oral exposure:

Maximum dose volume applied: 10 mL/kg bw

Doses:

1000, 1250, 1400, 1600, 1800, 2000, 2500 mg/kg bw

No. of animals per sex per dose:

1000, 1250, 1400 mg/kg bw: 5 males
1800, 2000 mg/kg bw: 5 females
1600, 2500 mg/kg bw: 5 males and 5 females

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of weighing: weekly
- Necropsy of survivors performed: yes, macroscopical examination
- Other examinations performed: clinical signs

Statistics:

LD50 Evaluation; 95% confidence interval

Results and discussion

Effect levels

Effect levels 1

Sex:

male

Dose descriptor:

LD50

Effect level:

1 320 mg/kg bw

Based on:

test mat.

95% CL:

841 - 1 550

Effect levels 2

Sex:

female

Dose descriptor:

LD50

Effect level:

1 700 mg/kg bw

Based on:

test mat.

95% CL:

961 - 2 040

Mortality:

1000 mg/kg bw: 0/5 male animals
1250 mg/kg bw: 2/5 male aninmals
1400 mg/kg bw: 3/5 male animals
1600 mg/kg bw: 5/5 male animals and 1/5 female animals
1800 mg/kg bw: 4/5 female animals
2000 mg/kg bw: 5/5 female animals
2500 mg/kg bw: 5/5 male animals and 5/5 female animals

Clinical signs:

other: Coat bristling, cowering posture, decreased activity, respiratory rate increased, irregular respiration, panting, gait stilted, forward crawling, trembling, prone position, paw reflex to pinching negative, palpebral fissure narrow, cear and bloody lacrima

Gross pathology:

Animals which died within the study:
liver and spleen brightend, spleen significantly reduced, gastrointestinal tract filled with dark blood.
Animals which survived:
no macroscopical findings.

Applicant's summary and conclusion

Conclusions:

Under the conditions of the present aucte oral toxicity study 2,4-Dichlorobenzotrichloride revealed a LD50 value of 1320 mg/kg bw for male and 1700 mg/kg bw for female animals.

Based on the results obtained from testing 2,4-Dichlorobenzotrichloride has to be classified as category 4, H302 (harmful if swallowed) according to Regulation (EC) No 1272/2008 (CLP).